Matches in SemOpenAlex for { <https://semopenalex.org/work/W2398097603> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2398097603 endingPage "377" @default.
- W2398097603 startingPage "374" @default.
- W2398097603 abstract "OBJECTIVE: To investigate the release and intracellular localization of high mobility group box chromosomal protein 1 (HMGB1) in the peripheral blood monocytes of rheumatoid arthritis (RA) patients and the inhibitive effect of thalidomide. METHODS: 19 RA patients and 20 healthy controls were included in the study. Monocytes were separated from peripheral blood with Ficoll density gradient centrifugation. Monocytes were treated with 100 ng/ml tumor necrosis factor alpha (TNFalpha) or 100 ng/ml TNFalpha plus 40 microg/ml thalidomide and grown in an incubator at 37 degrees C with 5% CO2 for 24 hours. The culture supernatants of the monocytes were collected. HMGB1 level in the culture medium was detected with Western blot. In addition, the intracellular localization of HMGB1 in the monocytes was investigated with immunocytochemical analysis. RESULTS: Without stimulation, the release of HMGB1 protein was significantly increased in the culture supernatants of peripheral blood monocytes from RA patients as compared with that from healthy controls (P < 0.05). TNFalpha (100 ng/ml) did not further increase the release of HMGB1 in the monocytes from the patients with RA. Thalidomide (40 microg/ml) could inhibit the release of HMGB1 in the monocytes from RA patients stimulated with TNFalpha (P < 0.05). In the monocytes from RA patients, HMGB1 was mainly localized in the nucleus. Treatment with TNFalpha (100 ng/ml) for 24 hours resulted in a cytoplasmic translocation of HMGB1, which was inhibited significantly by thalidomide. CONCLUSION: TNFalpha induces the release and cytoplasmic translocation of HMGB1 in the peripheral blood monocytes of RA patients and thalidomide inhibits the release and translocation of HMGB1." @default.
- W2398097603 created "2016-06-24" @default.
- W2398097603 creator A5014780429 @default.
- W2398097603 creator A5019486536 @default.
- W2398097603 creator A5021356497 @default.
- W2398097603 creator A5021737513 @default.
- W2398097603 creator A5079754796 @default.
- W2398097603 date "2008-05-01" @default.
- W2398097603 modified "2023-09-23" @default.
- W2398097603 title "The plasmic translocation and release of high mobility group box chromosomal protein 1 in peripheral blood monocytes of patients with rheumatoid arthritis and the effect of thalidomide" @default.
- W2398097603 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18953944" @default.
- W2398097603 hasPublicationYear "2008" @default.
- W2398097603 type Work @default.
- W2398097603 sameAs 2398097603 @default.
- W2398097603 citedByCount "1" @default.
- W2398097603 countsByYear W23980976032017 @default.
- W2398097603 crossrefType "journal-article" @default.
- W2398097603 hasAuthorship W2398097603A5014780429 @default.
- W2398097603 hasAuthorship W2398097603A5019486536 @default.
- W2398097603 hasAuthorship W2398097603A5021356497 @default.
- W2398097603 hasAuthorship W2398097603A5021737513 @default.
- W2398097603 hasAuthorship W2398097603A5079754796 @default.
- W2398097603 hasConcept C104317684 @default.
- W2398097603 hasConcept C126322002 @default.
- W2398097603 hasConcept C132946820 @default.
- W2398097603 hasConcept C134018914 @default.
- W2398097603 hasConcept C137061746 @default.
- W2398097603 hasConcept C138626823 @default.
- W2398097603 hasConcept C153911025 @default.
- W2398097603 hasConcept C17991360 @default.
- W2398097603 hasConcept C202751555 @default.
- W2398097603 hasConcept C203014093 @default.
- W2398097603 hasConcept C24998067 @default.
- W2398097603 hasConcept C2776364478 @default.
- W2398097603 hasConcept C2776914184 @default.
- W2398097603 hasConcept C2777575956 @default.
- W2398097603 hasConcept C2779609412 @default.
- W2398097603 hasConcept C2780545709 @default.
- W2398097603 hasConcept C2781184567 @default.
- W2398097603 hasConcept C5362183 @default.
- W2398097603 hasConcept C55493867 @default.
- W2398097603 hasConcept C71924100 @default.
- W2398097603 hasConcept C79879829 @default.
- W2398097603 hasConcept C86803240 @default.
- W2398097603 hasConceptScore W2398097603C104317684 @default.
- W2398097603 hasConceptScore W2398097603C126322002 @default.
- W2398097603 hasConceptScore W2398097603C132946820 @default.
- W2398097603 hasConceptScore W2398097603C134018914 @default.
- W2398097603 hasConceptScore W2398097603C137061746 @default.
- W2398097603 hasConceptScore W2398097603C138626823 @default.
- W2398097603 hasConceptScore W2398097603C153911025 @default.
- W2398097603 hasConceptScore W2398097603C17991360 @default.
- W2398097603 hasConceptScore W2398097603C202751555 @default.
- W2398097603 hasConceptScore W2398097603C203014093 @default.
- W2398097603 hasConceptScore W2398097603C24998067 @default.
- W2398097603 hasConceptScore W2398097603C2776364478 @default.
- W2398097603 hasConceptScore W2398097603C2776914184 @default.
- W2398097603 hasConceptScore W2398097603C2777575956 @default.
- W2398097603 hasConceptScore W2398097603C2779609412 @default.
- W2398097603 hasConceptScore W2398097603C2780545709 @default.
- W2398097603 hasConceptScore W2398097603C2781184567 @default.
- W2398097603 hasConceptScore W2398097603C5362183 @default.
- W2398097603 hasConceptScore W2398097603C55493867 @default.
- W2398097603 hasConceptScore W2398097603C71924100 @default.
- W2398097603 hasConceptScore W2398097603C79879829 @default.
- W2398097603 hasConceptScore W2398097603C86803240 @default.
- W2398097603 hasIssue "5" @default.
- W2398097603 hasLocation W23980976031 @default.
- W2398097603 hasLocation W23980976032 @default.
- W2398097603 hasOpenAccess W2398097603 @default.
- W2398097603 hasPrimaryLocation W23980976031 @default.
- W2398097603 hasRelatedWork W1277435932 @default.
- W2398097603 hasRelatedWork W1420073098 @default.
- W2398097603 hasRelatedWork W1561259918 @default.
- W2398097603 hasRelatedWork W1642137460 @default.
- W2398097603 hasRelatedWork W1974757124 @default.
- W2398097603 hasRelatedWork W2024708384 @default.
- W2398097603 hasRelatedWork W2028270739 @default.
- W2398097603 hasRelatedWork W2046122625 @default.
- W2398097603 hasRelatedWork W2076327691 @default.
- W2398097603 hasRelatedWork W2150954016 @default.
- W2398097603 hasRelatedWork W2319994965 @default.
- W2398097603 hasRelatedWork W2380128972 @default.
- W2398097603 hasRelatedWork W2396650917 @default.
- W2398097603 hasRelatedWork W2415487816 @default.
- W2398097603 hasRelatedWork W2519570907 @default.
- W2398097603 hasRelatedWork W2752062214 @default.
- W2398097603 hasRelatedWork W2766050429 @default.
- W2398097603 hasRelatedWork W3198113701 @default.
- W2398097603 hasRelatedWork W412092935 @default.
- W2398097603 hasRelatedWork W2764441907 @default.
- W2398097603 hasVolume "47" @default.
- W2398097603 isParatext "false" @default.
- W2398097603 isRetracted "false" @default.
- W2398097603 magId "2398097603" @default.
- W2398097603 workType "article" @default.