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- W2398256210 abstract "The objective of this study was to determine the effects of ionomycin combined with cytochalasin B (CB), cycloheximide (CHX), or 6-dimethylaminopurine (6-DMAP) on the activation of porcine oocytes. In Experiment 1, in vitro matured oocytes were activated with 15, 20, 25 or 30mmol L^(-1) ionomycin separately. Activation rates of 20, 25mmol L^(-1) and 30mmol L^(-1) treatments were higher (P<0.05) than that of 15mmol L^(-1) treatment. In Experiment 2, in vitro matured oocytes were activated with 20mmol L^(-1) ionomycin for 10, 20, 30, 40 or 50mim and then incubated with 2mmol L^(-1) 6-DMAP for 6h. Cleavage and blastocyst rates [(72.40±13.02)%, (25.37±11.43)%] after treatments for 40mim were higher (P>0.05) than those of the other treatments. In Experiment 3, matured oocytes were activated with ionomycin and then incubated with 7.5mg mL^(-1) CB, 10mg mL^(-1) CHX, 2mmol L^(-1) 6-DMAP, 7.5mg mL^(-1) CB+10mg mL^(-1) CHX or 7.5mg mL^(-1) CB+2mmol L^(-1) 6-DMAP for 6h. The rates of activation, cleavage and blastocyst of 2mmol L^(-1) 6-DMAP treatment [(86.05±4.29)%, (61.77±8.10)% and (21.62±3.31)%] were higher (P<0.05) than those of 7.5mg mL^(-1) CB treatment. In Experiment 4, matured oocytes were activated with ionomycin and then incubated with 2mmol L^(-1) 6-DMAP for 3.5, 5.5 or 7.5h. Cleavage rates and blastocyst rates of 5.5h treatment [(66.59±14.36)% and (25.40±10.l6)%] were higher (P>0.05) than those of other treatments. In conclusion, activation of porcine oocytes appears to be most successful using the combination of ionomycin (20mmol L^(-1), 40mim) followed by 6-DMAP (2mmol L^(-1), 5.5h)." @default.
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- W2398256210 date "2005-10-20" @default.
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- W2398256210 title "Effects of Chemical Activation on the Parthenogenetic Development of Porcine in vitro Maturation Oocytes" @default.
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