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- W2398642910 abstract "A sequential approach was developed to label tyrosine sulfate and peptides containing tyrosine sulfate selectively. Amino acids and peptides containing tyrosine and tyrosine sulfate were first iodinated using chloramine-T-method. Reaction products were determined by RP-HPLC. Mono- and biiodination of tyrosine and several model peptides was achieved within 120 s incubation time. Iodination of free tyrosine sulfate and sulfated cholecystokinin26-33 was less than 5%. After desulfation of the reaction products with 1 N HCl successful radioiodination of desulfated tyrosine was carried out whereas tyrosine did incorporate radioactive iodine only 10%. As shown by RP-HPLC specific labeling of tyrosine sulfate containing peptides with 125iodine was achieved." @default.
- W2398642910 created "2016-06-24" @default.
- W2398642910 date "1994-01-01" @default.
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- W2398642910 title "Short Communication" @default.
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- W2398642910 doi "https://doi.org/10.1515/bchm3.1994.375.3.201" @default.
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