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- W2399167493 abstract "The bindings of 3 kinds of aidehyde pollutants, formaldehyde, acetaldehyde, acrolein, with both prokaryotic and eukaryotic DNA, and their genotoxic effect and mechanism were conducted by the shifts of maximum UV absorption peak to determine binding effects and by HPLC to determine binding sites in vitro model system. The shifts of maximum UV absorption peak of prokaryotic DNA contaminated by 3 kinds of aldehyde pollutants are not significant; but after the DNA extracted from prokaryotic bacteria reacting with formaldehyde in tube, the shifts of maximum UV absorption peak of DNA are significant; the shifts of maximum UV absorption peak of eukaryotic DNA contaminated by 3 kinds of aldehyde pollutants are significant also. The reacition of acetaldehyde with dG reduced by NaBH4 was separated and detected by HPLC, the product was determined qualitatively as N2-ethaldeoxyguanosine adduct. The 3 kinds of aldehyde pollutants could bind with cellular DNA to express genotoxic effects; and the N2 site of deoxyguanosine is the possible covalent binding site." @default.
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- W2399167493 date "2001-01-01" @default.
- W2399167493 modified "2023-10-18" @default.
- W2399167493 title "[The bindings of typical aldehydes pollutants with cell DNA]." @default.
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