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• W2399222945 abstract "Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant protein production and engineering. One drawback of cell-free technology is the necessity to store the major components—cell extracts and energy systems—below freezing in bulky aqueous solutions. Here we describe simple methods for lyophilizing extracts and preparing powdered energy systems for CFPS. These techniques allow for high-density storage of cell-free systems that are more robust against temperature and bacterial degradation. Our methods have the potential to decrease storage expenses, allow for longer shelf-life of cell extracts at room temperature, and enable durable portable protein production technologies." @default.
• W2399222945 created "2016-06-24" @default.
• W2399222945 creator A5028584729 @default.
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• W2399222945 creator A5048221077 @default.
• W2399222945 creator A5089571546 @default.
• W2399222945 date "2014-04-01" @default.
• W2399222945 modified "2023-10-18" @default.
• W2399222945 title "Lyophilized <i>Escherichia coli</i>-based cell-free systems for robust, high-density, long-term storage" @default.
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• W2399222945 doi "https://doi.org/10.2144/000114158" @default.
• W2399222945 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24724844" @default.
• W2399222945 hasPublicationYear "2014" @default.
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