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• W2399494464 abstract "One of the more popular single-molecule approaches in biological science is single-molecule fluorescence microscopy, which will be the subject of the following section of this volume. Fluorescence methods provide the sensitivity required to study biology on the single-molecule level, but they also allow access to useful measurable parameters on time and length scales relevant for the biomolecular world. Before several detailed experimental approaches will be addressed, we will first give a general overview of single-molecule fluorescence microscopy. We start with discussing the phenomenon of fluorescence in general and the history of single-molecule fluorescence microscopy. Next, we will review fluorescent probes in more detail and the equipment required to visualize them on the single-molecule level. We will end with a description of parameters measurable with such approaches, ranging from protein counting and tracking, single-molecule localization super-resolution microscopy, to distance measurements with Förster Resonance Energy Transfer and orientation measurements with fluorescence polarization." @default.
• W2399494464 created "2016-06-24" @default.
• W2399494464 creator A5012397278 @default.
• W2399494464 creator A5043708959 @default.
• W2399494464 creator A5057415114 @default.
• W2399494464 date "2017-09-23" @default.
• W2399494464 modified "2023-09-24" @default.
• W2399494464 title "A Brief Introduction to Single-Molecule Fluorescence Methods" @default.
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• W2399494464 doi "https://doi.org/10.1007/978-1-4939-7271-5_5" @default.
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• W2399494464 hasPublicationYear "2017" @default.
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