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- W2399563732 abstract "Two kinds of murine hybridoma, one(CH07E02) against the colon cancer cell SC1 and the otheragainst the Chlamyida trachomatis (C.t.)L2 type, were constructed via cell fusion. The culture fluid of CH07E02 showed ELISA activity against SC1, but the question of antibody specificity against CEA, CA 19-9, or any other surface antigen of SC1 was not resolved. The supernatant from the anti C.t.L2 hybridoma culture showed cross-reactivity with C.t.G type cells with varying degrees of relative activity depending upon the particular hybridoma clones used. These two hybridomas and another one were cultivated in two types of bioreactors, i.e.the spinner culture flask and the Celligen bioreactor under various culture conditions, Kanamycin, the antibiotic used to protect the culture liquid from contamination, did not severely inhibit the growthe of hybridoma. In an effort to reduce the medium cost and to see whether there is any additional activating components a 3 to 7 mixture ofspent medium and fresh medium while holding the resulting fresh FCS concentration constaant at 7% was used with 7% FCS without spent medium addition as the control. The medium containing the spent medium added showed a simliar pattern of cell growth when the inoculum size was 2.0{times>10^5$7.0{times>10^4$ cell/ml one could see a definitely positive effect of adding spent medium in substantially reducing the lag period following the inoculation. To see the effect of shear rate on the hybridoma cluture the impeller of spinner flask was rotated at several different levels of rpm. The cell death rate continued to decrease as the rpm reduced to 80, the lowest value that could be achieved with the stirrer at hand, showing that the mechanical damage caused by the impeller agitation is a more determining factor despite the probable positive effect on oxygen transfer. At this low level of rpm the positive mass transfer effect from the single impeller will be minimal and a kind of surface aerator was installed at the interface between the air- gas phase and the liquid medium to give any possible enhancement in surface gas transfer. When the height of the liquid level inside the reactor was not that high, the cells after the maximum cell concentration was reached maintained the viability much longer than without surface aerator although there was not much change in the maximum cell concentration achievable. For culture experience with al still larger bioreactor, the Celligen of 2 liter volume with pH-and DO-control system was utilized. One run at pH= and DO= demonstrated the maintenance of cell viability for a substantial period of time." @default.
- W2399563732 created "2016-06-24" @default.
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- W2399563732 date "1988-01-01" @default.
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- W2399563732 title "Effect of Spent Medium and Stirring on the Hybridoma Growth and Monoclonal Antibody Production in Spinner Flask and Celligen Bioreactor" @default.
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