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- W2399683571 abstract "Potassium channels conduct K+ ions selectively and at very high rates. Central to the function of K+ channels is a structural unit called the selectivity filter. In the selectivity filter, a row of four K+ binding sites are created using mainly the backbone carbonyl oxygen atoms. Due to the involvement of the protein backbone, site-directed mutagenesis is of limited utility in investigating the selectivity filter. In order to overcome this limitation, we have developed a semisynthetic approach, which permits the use of chemical synthesis to manipulate the selectivity filter. In this chapter, we describe the protocols that we have developed for the semisynthesis of the K+ channel, KcsA. We anticipate that the protocols described in this chapter will also be applicable for the semisynthesis of other integral membrane proteins of interest." @default.
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- W2399683571 date "2013-01-01" @default.
- W2399683571 modified "2023-09-26" @default.
- W2399683571 title "Engineering K+ Channels Using Semisynthesis" @default.
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- W2399683571 doi "https://doi.org/10.1007/978-1-62703-345-9_1" @default.
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