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• W2399685597 abstract "AbstractHuman fetal mesenchymal stem cells (hfMSC) demonstrate extensive expansion and differentiation capacities and are hence being studied for use in stem cell therapeutics, including gene delivery. With advanced prenatal diagnosis, fetal gene therapy represents an additional avenue for the treatment of inherited deficiencies. We have recently demonstrated harvesting of first-trimester fMSC from fetal blood for ex vivo genetic engineering to introduce genes of interest, and finally intra-uterine transplantation (IUT) of these cells to the fetus. Here we discuss methods in the harvesting of hfMSC, lentiviral transduction to introduce genes of interest, and in vitro methods to characterise transgene expression.Key wordsHuman fetal mesenchymal stem cellsEx vivo transductionFluorescence microscopyFlow cytometryOsteogenic inductionAdipogenic inductionChondrogenic induction" @default.
• W2399685597 created "2016-06-24" @default.
• W2399685597 creator A5030659181 @default.
• W2399685597 creator A5059252106 @default.
• W2399685597 date "2009-11-19" @default.
• W2399685597 modified "2023-10-12" @default.
• W2399685597 title "Lentiviral Vector Transduction of Fetal Mesenchymal Stem Cells" @default.
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• W2399685597 doi "https://doi.org/10.1007/978-1-60761-533-0_9" @default.
• W2399685597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20225041" @default.
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