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• W2399690963 abstract "This chapter describes a system using adeno-associated viral (AAV) vector to deliver hypoxia-inducible gene expression to ischemic hearts. The hypoxia induction of gene expression in this system is based on the accumulation of hypoxia-inducible factor-1 (HIF-1) in ischemic hearts and the use of hypoxia-response element (HRE) identified from the enhancers of genes, the expression of which can be induced by hypoxia. The methods of plasmid and AAV vector construction for hypoxia-inducible gene expression, viral vector production and purification, and viral titer determination are described. This chapter also illustrates the methods that can be used to test hypoxia-inducible gene expression in vitro and in vivo, including hypoxia treatment of cultured cells, generation of murine ischemic heart models, and analysis of gene expression." @default.
• W2399690963 created "2016-06-24" @default.
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• W2399690963 creator A5015169877 @default.
• W2399690963 date "2007-01-01" @default.
• W2399690963 modified "2023-09-26" @default.
• W2399690963 title "Adeno-Associated Viral Vector-Delivered Hypoxia-Inducible Gene Expression in Ischemic Hearts" @default.
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• W2399690963 doi "https://doi.org/10.1007/978-1-59745-030-0_19" @default.
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