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- W2399691208 abstract "We have isolated a transposable element, designated PAT-1, from the free-living nematode Panagrellus redivivus. P. redivivus strain C1S was found to have a high spontaneous mutation frequency compared to the standard Caenorhabditis elegans laboratory strain N2. To characterize the genetic lesions occurring in spontaneous C15 mutants, we molecularly cloned the homolog of the C. elegans unc-22 gene from wild-type P. redivivus and two strains carrying sponta- neous mutations in this gene. One of these mutations resulted from the insertion of a 4.8-kilobase segment of repetitive DNA. This repetitive element (PAT-1) varies in copy number (10-50 copies) and location in different P. redivivus strains and is absent from C. elegans. The element could be useful as a transformation vector for C. elegans. Our approach is a general one that could be used to isolate additional nematode trans- posons from other species." @default.
- W2399691208 created "2016-06-24" @default.
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- W2399691208 date "2016-01-01" @default.
- W2399691208 modified "2023-09-26" @default.
- W2399691208 title "Isolation and characterization of a nematode transposable element from Panagrellus redivivus" @default.
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