FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W239976209> ?p ?o ?g. }

• W239976209 endingPage "938" @default.
• W239976209 startingPage "933" @default.
• W239976209 abstract "Research Article1 March 1989free access Identifying functional regions of rRNA by insertion mutagenesis and complete gene replacement in Tetrahymena thermophila. R. Sweeney R. Sweeney Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104. Search for more papers by this author M. C. Yao M. C. Yao Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104. Search for more papers by this author R. Sweeney R. Sweeney Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104. Search for more papers by this author M. C. Yao M. C. Yao Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104. Search for more papers by this author Author Information R. Sweeney1 and M. C. Yao1 1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104. The EMBO Journal (1989)8:933-938https://doi.org/10.1002/j.1460-2075.1989.tb03454.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The free, linear macronuclear ribosomal RNA genes (rDNA) of Tetrahymena are derived from a unique copy of micronuclear rDNA during development. We have injected cloned copies of the micronuclear rDNA that have been altered in vitro into developing macronuclei and obtained transformants that express the paromomycin-resistant phenotype specified by the injected rDNA. In most cases, these transformants contain almost exclusively the injected rDNA which has been accurately processed into macronuclear rDNA. Mutants with a 119 bp insertion at three points in the transcribed spacers and at two points in the 26S rRNA coding region were tested. Cells containing these spacer mutant rDNAs are viable, although one of them grows slowly. This slow-growing line contains the insertion between the 5.8S and 26S rRNA coding regions and accumulates more rRNA processing intermediates than control lines. One of the 26S rRNA mutants failed to generate transformants, but the other did. These transformants grew normally, and produced 26S rRNA containing the inserted sequence. A longer insertion (2.3 kb) at the same four points either abolished transformation or generated transformants that retained at least some wild-type rDNA. This study reveals that some rRNA sequences can be altered without significantly affecting cell growth. Previous ArticleNext Article Volume 8Issue 31 March 1989In this issue RelatedDetailsLoading ..." @default.
• W239976209 created "2016-06-24" @default.
• W239976209 creator A5005946897 @default.
• W239976209 creator A5063850772 @default.
• W239976209 date "1989-03-01" @default.
• W239976209 modified "2023-09-30" @default.
• W239976209 title "Identifying functional regions of rRNA by insertion mutagenesis and complete gene replacement in Tetrahymena thermophila." @default.
• W239976209 cites W1463780262 @default.
• W239976209 cites W1491607388 @default.
• W239976209 cites W1790030561 @default.
• W239976209 cites W1797446807 @default.
• W239976209 cites W1964061683 @default.
• W239976209 cites W1967559077 @default.
• W239976209 cites W1968238933 @default.
• W239976209 cites W1972555985 @default.
• W239976209 cites W1979151071 @default.
• W239976209 cites W1985677555 @default.
• W239976209 cites W1991346121 @default.
• W239976209 cites W1994053857 @default.
• W239976209 cites W1995218232 @default.
• W239976209 cites W2000764465 @default.
• W239976209 cites W2009222089 @default.
• W239976209 cites W2011706888 @default.
• W239976209 cites W2021248436 @default.
• W239976209 cites W2028600064 @default.
• W239976209 cites W2029987769 @default.
• W239976209 cites W2056063068 @default.
• W239976209 cites W2061368671 @default.
• W239976209 cites W2063025555 @default.
• W239976209 cites W2063450941 @default.
• W239976209 cites W2077077811 @default.
• W239976209 cites W2083350289 @default.
• W239976209 cites W2084988722 @default.
• W239976209 cites W2114380066 @default.
• W239976209 cites W2114832888 @default.
• W239976209 doi "https://doi.org/10.1002/j.1460-2075.1989.tb03454.x" @default.
• W239976209 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/400893" @default.
• W239976209 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2542027" @default.
• W239976209 hasPublicationYear "1989" @default.
• W239976209 type Work @default.
• W239976209 sameAs 239976209 @default.
• W239976209 citedByCount "46" @default.
• W239976209 countsByYear W2399762092015 @default.
• W239976209 countsByYear W2399762092016 @default.
• W239976209 countsByYear W2399762092018 @default.
• W239976209 crossrefType "journal-article" @default.
• W239976209 hasAuthorship W239976209A5005946897 @default.
• W239976209 hasAuthorship W239976209A5063850772 @default.
• W239976209 hasBestOaLocation W2399762091 @default.
• W239976209 hasConcept C104317684 @default.
• W239976209 hasConcept C153911025 @default.
• W239976209 hasConcept C16318435 @default.
• W239976209 hasConcept C207812311 @default.
• W239976209 hasConcept C2777166614 @default.
• W239976209 hasConcept C501734568 @default.
• W239976209 hasConcept C54355233 @default.
• W239976209 hasConcept C67905577 @default.
• W239976209 hasConcept C86803240 @default.
• W239976209 hasConcept C90132467 @default.
• W239976209 hasConceptScore W239976209C104317684 @default.
• W239976209 hasConceptScore W239976209C153911025 @default.
• W239976209 hasConceptScore W239976209C16318435 @default.
• W239976209 hasConceptScore W239976209C207812311 @default.
• W239976209 hasConceptScore W239976209C2777166614 @default.
• W239976209 hasConceptScore W239976209C501734568 @default.
• W239976209 hasConceptScore W239976209C54355233 @default.
• W239976209 hasConceptScore W239976209C67905577 @default.
• W239976209 hasConceptScore W239976209C86803240 @default.
• W239976209 hasConceptScore W239976209C90132467 @default.
• W239976209 hasIssue "3" @default.
• W239976209 hasLocation W2399762091 @default.
• W239976209 hasLocation W2399762092 @default.
• W239976209 hasLocation W2399762093 @default.
• W239976209 hasOpenAccess W239976209 @default.
• W239976209 hasPrimaryLocation W2399762091 @default.
• W239976209 hasRelatedWork W1920751942 @default.
• W239976209 hasRelatedWork W2002948976 @default.
• W239976209 hasRelatedWork W2009647017 @default.
• W239976209 hasRelatedWork W2067243745 @default.
• W239976209 hasRelatedWork W2076061307 @default.
• W239976209 hasRelatedWork W2084739049 @default.
• W239976209 hasRelatedWork W2093056240 @default.
• W239976209 hasRelatedWork W2094337193 @default.
• W239976209 hasRelatedWork W2133882122 @default.
• W239976209 hasRelatedWork W2135257384 @default.
• W239976209 hasVolume "8" @default.
• W239976209 isParatext "false" @default.
• W239976209 isRetracted "false" @default.
• W239976209 magId "239976209" @default.
• W239976209 workType "article" @default.