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- W2399805067 abstract "Banana is one of the most important fruit crops in Egypt both for domestic and export markets. At present, the industry is beset with low productivity due to Banana bunchy top virus (BBTV) and Banana mosaic virus (BMV). Banana cultivars are not appropriate for sexual breeding because both male and female are sterile. Thus, BBTV and BMV resistance cannot be incorporated into the banana by conventional breeding. Use of biotechnology techniques combined with mutagenesis using physical mutagens in Musa spp. allows genetic variability to be increased to improve important agronomic characters. The objective of this study was to develop mutant clones of Musa cv. ‘Grand-Nain’ with reduced height, good agronomical traits and resistance to both of BBTV and BMV viruses. Multiple buds obtained by in vitro culture were subjected to gamma radiation from 60 Co sources at 20, 40 and 60 Gy. Irradiated buds were multiplied, elongated, rooted, hardened and acclimatized in the glasshouse and were transplanted to the field. The selection of mutant plants is based on morphological and ISSR-PCR markers. DNA-based markers were reliable and reproducible for mutant selection of BBTV- and BMVresistant banana plants used in this study. Field screening was carried out against two different isolates of BBTV and BMV using a syringe method of inoculation. Only three banana plants treated with 60 Gy were resistant to the BMV isolate. No BBTV-resistant banana plants were obtained." @default.
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- W2399805067 date "2011-01-01" @default.
- W2399805067 modified "2023-09-26" @default.
- W2399805067 title "Mutation Breeding of Banana cv. 'Grand-Nain' for Resistance to Some Banana Viruses Using Biotechnology and Physical Mutagens" @default.
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