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- W2400027250 abstract "9507 Background: Current standards in pathological diagnosis are based on tissue of origin, microscopic examination, immunohistochemistry and immunophenotyping. This leads to a most likely diagnosis utilizing minimal acceptable resources, ignoring potentially important information based upon likelihood of occurrence. Methods: We have developed a novel method for analyzing heterogeneous clinical samples utilizing a cellular microarray to profile both cell molecular composition and cellular function in a rapid fashion. By co-spotting mixtures of a) capture probes, which recognize cell surface molecules, b) detection probes, which recognize molecules secreted by cells and c) effector probes, which can signal a change in cell behavior, in a microarray format, cells can be specifically captured and analyzed for their molecular and behavioral patterns. Results: Blood, lymph nodes and surgical biopsies from patients with malignancies were dissociated into single cell suspensions and applied to the cellular microarray. Diagnosis of the tumor type was obtained by analysis of a molecular profile. Benign and malignant cells were distinguishable by differences in their microscopic appearance, glucose metabolism, and their metabolic capacity. Functional profiling of secreted factors such as vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMPS), provided a specific molecular signature. The cellular microarray also determined the presence of unexpected therapeutic targets, such as overexpression of c-kit, on the surface of tumor cells from some of the clinical samples. Conclusions: The use of cellular microarrays provides a versatile, simple and rapid platform for profiling molecular and functional patterns in single cells. This information is correlated to clinical disease, and may provide improved ability to determine best treatment, identification of unexpected therapeutic options, and provide more accurate prognosis. This technology may be applied to routine clinical laboratory analysis and lead to the development of functional pathology using viable cellular samples. No significant financial relationships to disclose." @default.
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- W2400027250 date "2004-07-15" @default.
- W2400027250 modified "2023-09-25" @default.
- W2400027250 title "Functional and molecular profiling of heterogeneous tumor samples using a novel cellular microarray" @default.
- W2400027250 doi "https://doi.org/10.1200/jco.2004.22.90140.9507" @default.
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