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- W2400194655 abstract "The influence of sampling issues on the reproducibility of volume-weighted mean nuclear volume (mean v) and mean nuclear area (MNA) assessments in patients with International Federation of Gynecology and Obstetrics stage III and IV ovarian carcinoma was evaluated. Ten cases representing the whole range of MNA values were selected from a population of 131 cases. The MNA and mean v of the same tumor cell nuclei were determined in one session by switching between the stereologic module and the morphometric module of the video overlay program used. For both MNA and mean v in one series of measurements, tumor nuclei were sampled from the whole tumor area and in a second series from the most poorly differentiated part (the measurement area) in each section, thus giving four series of measurements per case. For all four series, 500 nuclei were point sampled from approximately 100 systematically randomly selected fields of vision, using the automated scanning stage controlled by the morphometry program. These large samples, containing 500 nuclei for each case, were regarded as representative in each case. To investigate the susceptibility of MNA and mean v to variance at lower sampling levels (fields, nuclei), a nested analysis of variance was performed. Then the influence of sample size and sampling method was evaluated by drawing subsets from these 500 nuclei in each case in three different ways (cluster, systematic or random) with four different sample sizes (50, 100, 125, 250). It was shown that for MNA assessed in the measurement area, the variance between patients contributed the most to the total variance.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
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- W2400194655 date "1995-08-01" @default.
- W2400194655 modified "2023-10-18" @default.
- W2400194655 title "Volume-weighted mean nuclear volume and nuclear area in advanced ovarian carcinoma. An investigation of sampling methods, sample size and reproducibility." @default.
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