FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2400464694> ?p ?o ?g. }

• W2400464694 endingPage "83" @default.
• W2400464694 startingPage "65" @default.
• W2400464694 abstract "Two-dimensional [1H, 13C] heteronuclear single quantum correlation (HSQC) spectroscopy nuclear magnetic resonance (NMR) is a comprehensive tool in metabolic flux analysis using 13C-labeling experiments. NMR is particularly relevant when extensive isotopomer measurements are required, such as for plant cells and tissues, which contain multiple cellular compartments. Several isotope isomers (isotopomers) can be detected and their distribution extracted quantitatively from a single 2-D HSQC NMR spectrum. For example, 2-D HSQC detects the labeling patterns of adjacent carbon atoms and provides the enrichment of individual carbon atoms of the amino acids and glucosyl and mannosyl units present in hydrolysates of glycosylated protein. The HSQC analysis can quantitatively distinguish differences between the glucosyl units in the starch hydrolysate and a protein hydrolysate of plant biomass: this specifies crucial information about compartmentalization in the plant system. The peak structures obtained from the HSQC experiment show multiplet patterns that are directly related to the isotopomer abundances. These abundances have a nonlinear relationship to the fluxes via isotopomer balancing. Fluxes are obtained from the numerical solution of these balances and a stoichiometric model that includes biomass composition data as well as consumption rates of carbohydrate and nitrogen sources. Herein, we describe the methods for the experimental measurements for flux analysis, i.e., determination of the biomass composition (lipid, protein, soluble sugar, and starch) as well as detailed procedures of acid hydrolysis of protein and starch samples and NMR sample preparation, using soybean embryo culture as the model plant system. Techniques to obtain the relative intensity of 16 amino acids and glucosyl units for protein hydrolysate and the glucosyl units of starch hydrolysate of soybean embryos in 2-D HSQC NMR spectra also are provided." @default.
• W2400464694 created "2016-06-24" @default.
• W2400464694 creator A5017753088 @default.
• W2400464694 creator A5051137187 @default.
• W2400464694 creator A5076344070 @default.
• W2400464694 date "2013-10-10" @default.
• W2400464694 modified "2023-09-23" @default.
• W2400464694 title "Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance" @default.
• W2400464694 cites W1576233366 @default.
• W2400464694 cites W1767127367 @default.
• W2400464694 cites W1964292815 @default.
• W2400464694 cites W1965118188 @default.
• W2400464694 cites W1990712597 @default.
• W2400464694 cites W1992170087 @default.
• W2400464694 cites W2001449103 @default.
• W2400464694 cites W2010441870 @default.
• W2400464694 cites W2012791986 @default.
• W2400464694 cites W2018856264 @default.
• W2400464694 cites W2022768923 @default.
• W2400464694 cites W2024567167 @default.
• W2400464694 cites W2032271434 @default.
• W2400464694 cites W2035628607 @default.
• W2400464694 cites W2038070550 @default.
• W2400464694 cites W2045593084 @default.
• W2400464694 cites W2049167322 @default.
• W2400464694 cites W205459028 @default.
• W2400464694 cites W2057367356 @default.
• W2400464694 cites W2069734146 @default.
• W2400464694 cites W2081052511 @default.
• W2400464694 cites W2082056771 @default.
• W2400464694 cites W2082176639 @default.
• W2400464694 cites W2083364998 @default.
• W2400464694 cites W2087383714 @default.
• W2400464694 cites W2095386536 @default.
• W2400464694 cites W2104826241 @default.
• W2400464694 cites W2110601889 @default.
• W2400464694 cites W2114206363 @default.
• W2400464694 cites W2122483634 @default.
• W2400464694 cites W2138964739 @default.
• W2400464694 cites W2144560876 @default.
• W2400464694 cites W2145199186 @default.
• W2400464694 cites W2169674481 @default.
• W2400464694 cites W2395534694 @default.
• W2400464694 doi "https://doi.org/10.1007/978-1-62703-661-0_6" @default.
• W2400464694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24218211" @default.
• W2400464694 hasPublicationYear "2013" @default.
• W2400464694 type Work @default.
• W2400464694 sameAs 2400464694 @default.
• W2400464694 citedByCount "8" @default.
• W2400464694 countsByYear W24004646942014 @default.
• W2400464694 countsByYear W24004646942015 @default.
• W2400464694 countsByYear W24004646942016 @default.
• W2400464694 countsByYear W24004646942017 @default.
• W2400464694 countsByYear W24004646942018 @default.
• W2400464694 countsByYear W24004646942021 @default.
• W2400464694 crossrefType "book-chapter" @default.
• W2400464694 hasAuthorship W2400464694A5017753088 @default.
• W2400464694 hasAuthorship W2400464694A5051137187 @default.
• W2400464694 hasAuthorship W2400464694A5076344070 @default.
• W2400464694 hasConcept C113196181 @default.
• W2400464694 hasConcept C127124265 @default.
• W2400464694 hasConcept C160941953 @default.
• W2400464694 hasConcept C178790620 @default.
• W2400464694 hasConcept C185592680 @default.
• W2400464694 hasConcept C193125475 @default.
• W2400464694 hasConcept C19549132 @default.
• W2400464694 hasConcept C28800991 @default.
• W2400464694 hasConcept C32909587 @default.
• W2400464694 hasConcept C43617362 @default.
• W2400464694 hasConcept C529335014 @default.
• W2400464694 hasConcept C55493867 @default.
• W2400464694 hasConcept C62231903 @default.
• W2400464694 hasConcept C68709404 @default.
• W2400464694 hasConcept C71240020 @default.
• W2400464694 hasConcept C94412978 @default.
• W2400464694 hasConceptScore W2400464694C113196181 @default.
• W2400464694 hasConceptScore W2400464694C127124265 @default.
• W2400464694 hasConceptScore W2400464694C160941953 @default.
• W2400464694 hasConceptScore W2400464694C178790620 @default.
• W2400464694 hasConceptScore W2400464694C185592680 @default.
• W2400464694 hasConceptScore W2400464694C193125475 @default.
• W2400464694 hasConceptScore W2400464694C19549132 @default.
• W2400464694 hasConceptScore W2400464694C28800991 @default.
• W2400464694 hasConceptScore W2400464694C32909587 @default.
• W2400464694 hasConceptScore W2400464694C43617362 @default.
• W2400464694 hasConceptScore W2400464694C529335014 @default.
• W2400464694 hasConceptScore W2400464694C55493867 @default.
• W2400464694 hasConceptScore W2400464694C62231903 @default.
• W2400464694 hasConceptScore W2400464694C68709404 @default.
• W2400464694 hasConceptScore W2400464694C71240020 @default.
• W2400464694 hasConceptScore W2400464694C94412978 @default.
• W2400464694 hasLocation W24004646941 @default.
• W2400464694 hasLocation W24004646942 @default.
• W2400464694 hasOpenAccess W2400464694 @default.
• W2400464694 hasPrimaryLocation W24004646941 @default.
• W2400464694 hasRelatedWork W1980661851 @default.
• W2400464694 hasRelatedWork W1995820735 @default.
• W2400464694 hasRelatedWork W2027024068 @default.

• next