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• W2400502477 abstract "Objective To isolate and culture tumor stem cells from glioma tissues obtained at surgical operation and to study their biological characteristics. Methods Glioma tissues obtained from surgically resected specimens of 8 patients were fully chopped, trypsinized, and filtered to prepare single cell suspensions. The cells were cultured in serum-free medium with EGF, LIF and bFGF. CD133^＋ cells were purified by magnetic cell sorting, and cultured continuously in vitro to obtain tumor cell spheres. Tumor stem cells of the 5th passage were induced to differentiate with 10% FBS, and expression of cell differentiation markers such as Nestin, MAF2, GFAP was evaluated with immunocytochemistry techniques. Results CD133^＋ cells were successfully separated and cultured from one anasplastic mixed astrocyte-ependymocyte type glioma specimen. These cells maintained a sphere-like growth status in vitro （3 months, 14 passages）, and can self-renew, proliferate and conditionally differentiate into MAP2^＋ and GFAP^＋ cells. However, CD133^- cells did not posses these properties. Conclusion Glioma tissue contains tumor stem cells. Those cells can be cultured and passaged in vitro for a long term, and therefore to offer new approaches for studying cellular and molecular biology of glioma.Key words: Tumor stem cell;  Glioma;  Immunocytochemistry;  In vitro culture" @default.
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• W2400502477 date "2006-05-01" @default.
• W2400502477 modified "2023-09-27" @default.
• W2400502477 title "Isolation and culture of tumor stem cells from human brain glioma tissues" @default.
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