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- W2400565571 endingPage "258" @default.
- W2400565571 startingPage "245" @default.
- W2400565571 abstract "Correlative light and electron microscopy (CLEM) has become a powerful tool in life sciences. Particularly cryo-CLEM, the combination of fluorescence cryo-microscopy (cryo-FM) permitting for non-invasive specific multi-colour labelling, with electron cryo-microscopy (cryo-EM) providing the undisturbed structural context at a resolution down to the Ångstrom range, has enabled a broad range of new biological applications. Imaging rare structures or events in crowded environments, such as inside a cell, requires specific fluorescence-based information for guiding cryo-EM data acquisition and/or to verify the identity of the structure of interest. Furthermore, cryo-CLEM can provide information about the arrangement of specific proteins in the wider structural context of their native nano-environment. However, a major obstacle of cryo-CLEM currently hindering many biological applications is the large resolution gap between cryo-FM (typically in the range of ∼400 nm) and cryo-EM (single nanometre to the Ångstrom range). Very recently, first proof of concept experiments demonstrated the feasibility of super-resolution cryo-FM imaging and the correlation with cryo-EM. This opened the door towards super-resolution cryo-CLEM, and thus towards direct correlation of structural details from both imaging modalities." @default.
- W2400565571 created "2016-06-24" @default.
- W2400565571 creator A5053208117 @default.
- W2400565571 creator A5065111688 @default.
- W2400565571 creator A5068285707 @default.
- W2400565571 creator A5077882341 @default.
- W2400565571 date "2016-06-22" @default.
- W2400565571 modified "2023-10-17" @default.
- W2400565571 title "Towards correlative super-resolution fluorescence and electron cryo-microscopy" @default.
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