Matches in SemOpenAlex for { <https://semopenalex.org/work/W2400713443> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2400713443 endingPage "683" @default.
- W2400713443 startingPage "676" @default.
- W2400713443 abstract "Objective In order to investigate the distribution of Shiga toxin-producing Escherichia coli (STEC) among healthy sheep in a farm and the pathogenicity to mice and Vero cells of these STEC isolates. Methods We used polymerase chain reaction (PCR) to detect genes of eaeA, stx1, stx2, hlyA, which had been developed in this laboratory previously, combing the selective cultivation and Chrom-Agar (CA) O157 plates to isolate STEC strains. Results A total of 107 STEC strains were isolated in a sheep farm during six visits from August, 2008 to January, 2009. The isolation rate was 19.8% (107/550). These isolates belonged to 41 O serotypes and 60 O: H serotypes, except that 21 were O non-typable and 1 was rough. 093 was the common serotype. Some isolates such as O5, O91, O103, which are reported in other countries originated from healthy sheep were also isolated in this study. Stx2 positive rate was higher than that of stxl. 50% lethal dose assay in mice indicated the pathogenicity of isolates was low and none of the 3 tested isolates caused mice death. We selected 107 stx gene positive STEC strains to induce the lambdoid bacteriophages. The results showed that 71 out of 107 isolates formed plagues, while 28 did not after induction. Detection of Shiga toxins for three tested isolates in Vero cell assay indicated one stx gene positive strain lacked the toxigenicity to Vero cells. Conclusions Sheep are the natural reservoirs of STEC and they are healthy to carry STEC. Although these STEC isolates experience low pathogenicity to mice, they are potential threat to human health. Shiga toxin gene positive were not equal to production of Shiga toxins, so we need to further study the Shiga toxin expression and regulation mechanism." @default.
- W2400713443 created "2016-06-24" @default.
- W2400713443 creator A5019322293 @default.
- W2400713443 creator A5050837761 @default.
- W2400713443 creator A5054735097 @default.
- W2400713443 creator A5054806035 @default.
- W2400713443 creator A5068161836 @default.
- W2400713443 creator A5082437367 @default.
- W2400713443 date "2011-05-01" @default.
- W2400713443 modified "2023-09-23" @default.
- W2400713443 title "[Epidemiological investigation of Shiga toxin-producing Escherichia coli isolates originated from healthy sheep in one farm of Jiangsu Province and their pathogenicity]." @default.
- W2400713443 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21800631" @default.
- W2400713443 hasPublicationYear "2011" @default.
- W2400713443 type Work @default.
- W2400713443 sameAs 2400713443 @default.
- W2400713443 citedByCount "0" @default.
- W2400713443 crossrefType "journal-article" @default.
- W2400713443 hasAuthorship W2400713443A5019322293 @default.
- W2400713443 hasAuthorship W2400713443A5050837761 @default.
- W2400713443 hasAuthorship W2400713443A5054735097 @default.
- W2400713443 hasAuthorship W2400713443A5054806035 @default.
- W2400713443 hasAuthorship W2400713443A5068161836 @default.
- W2400713443 hasAuthorship W2400713443A5082437367 @default.
- W2400713443 hasConcept C10389963 @default.
- W2400713443 hasConcept C104317684 @default.
- W2400713443 hasConcept C105702510 @default.
- W2400713443 hasConcept C159047783 @default.
- W2400713443 hasConcept C2522874641 @default.
- W2400713443 hasConcept C2777367657 @default.
- W2400713443 hasConcept C2778022156 @default.
- W2400713443 hasConcept C2778411228 @default.
- W2400713443 hasConcept C2781001907 @default.
- W2400713443 hasConcept C49105822 @default.
- W2400713443 hasConcept C54355233 @default.
- W2400713443 hasConcept C547475151 @default.
- W2400713443 hasConcept C64502627 @default.
- W2400713443 hasConcept C86803240 @default.
- W2400713443 hasConcept C89138240 @default.
- W2400713443 hasConcept C89423630 @default.
- W2400713443 hasConceptScore W2400713443C10389963 @default.
- W2400713443 hasConceptScore W2400713443C104317684 @default.
- W2400713443 hasConceptScore W2400713443C105702510 @default.
- W2400713443 hasConceptScore W2400713443C159047783 @default.
- W2400713443 hasConceptScore W2400713443C2522874641 @default.
- W2400713443 hasConceptScore W2400713443C2777367657 @default.
- W2400713443 hasConceptScore W2400713443C2778022156 @default.
- W2400713443 hasConceptScore W2400713443C2778411228 @default.
- W2400713443 hasConceptScore W2400713443C2781001907 @default.
- W2400713443 hasConceptScore W2400713443C49105822 @default.
- W2400713443 hasConceptScore W2400713443C54355233 @default.
- W2400713443 hasConceptScore W2400713443C547475151 @default.
- W2400713443 hasConceptScore W2400713443C64502627 @default.
- W2400713443 hasConceptScore W2400713443C86803240 @default.
- W2400713443 hasConceptScore W2400713443C89138240 @default.
- W2400713443 hasConceptScore W2400713443C89423630 @default.
- W2400713443 hasIssue "5" @default.
- W2400713443 hasLocation W24007134431 @default.
- W2400713443 hasLocation W24007134432 @default.
- W2400713443 hasOpenAccess W2400713443 @default.
- W2400713443 hasPrimaryLocation W24007134431 @default.
- W2400713443 hasRelatedWork W1954900866 @default.
- W2400713443 hasRelatedWork W1986731002 @default.
- W2400713443 hasRelatedWork W2003201055 @default.
- W2400713443 hasRelatedWork W2007733050 @default.
- W2400713443 hasRelatedWork W2027078218 @default.
- W2400713443 hasRelatedWork W2035317540 @default.
- W2400713443 hasRelatedWork W2040924858 @default.
- W2400713443 hasRelatedWork W2063208338 @default.
- W2400713443 hasRelatedWork W2064023720 @default.
- W2400713443 hasRelatedWork W2070316491 @default.
- W2400713443 hasRelatedWork W2085016479 @default.
- W2400713443 hasRelatedWork W2086731593 @default.
- W2400713443 hasRelatedWork W2101434596 @default.
- W2400713443 hasRelatedWork W2116434034 @default.
- W2400713443 hasRelatedWork W2137969745 @default.
- W2400713443 hasRelatedWork W2170996280 @default.
- W2400713443 hasRelatedWork W2171745921 @default.
- W2400713443 hasRelatedWork W2333754558 @default.
- W2400713443 hasRelatedWork W2415446231 @default.
- W2400713443 hasRelatedWork W2794537767 @default.
- W2400713443 hasVolume "51" @default.
- W2400713443 isParatext "false" @default.
- W2400713443 isRetracted "false" @default.
- W2400713443 magId "2400713443" @default.
- W2400713443 workType "article" @default.