FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2400720319> ?p ?o ?g. }

• W2400720319 abstract "Introduction: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive cancer that results from the malignant transformation of T-cell precursors and affects children, adolescents and adults. In T-ALL, genetic lesions in several possible oncogenes and tumor suppressors have been shown to cooperatively contribute to leukemogenesis. The TLX1 (T-cell leukemia homeobox protein-1, HOX11) oncoprotein is aberrantly expressed in in 5-10% of pediatric patients and 30% of adult T-ALL patients due to chromosomal translocations. Although many downstream protein coding targets genes of TLX1 have been identified, the non-coding network downstream of TLX1 remains elusive. In this study we expand the TLX1 regulome towards long non-coding RNAs (lncRNAs). Experimental procedures: We measured the transcriptional response of all protein coding genes and lncRNAs following TLX1 knock down in the ALL-SIL cell line by polyA and total RNA-sequencing. In addition, similar mRNA-lncRNA expression profiles of 64 primary T-ALL patient samples were generated which included five TLX1+ cases. To establish the direct transcriptional TLX1 targets, we generated TLX1 and H3K27ac ChIP-sequencing data from ALL-SIL leukemic cells. Results: We confirm direct regulation of previously established protein coding gene targets and de novo TLX1 motif discovery also identified RUNX1 as an important mediator of the global TLX1 transcriptional network (Della-Gatta et al., Nature Medicine, 2012). Complementary to these data, our analysis for the first time establishes the TLX1 driven lncRNAome in thymocyte derived leukemic cells. Remarkably, the majority of TLX1 controlled lncRNAs were upregulated suggesting that they may be implicated in the TLX1 driven repression of protein coding gene expression. Notably, an important subset of these candidates is clearly associated with H3K27ac marked super-enhancer regions. Finally, pairwise mRNA-lncRNA correlation analysis allowed functional annotation of TLX1 targeted lncRNAs. To functionally interrogate candidate TLX1 regulated lncRNAs, LNA-mediated lncRNA knockdown experiments are currently performed as well as 4C-seq to explore the regulatory interactions in which these lncRNAs are involved. Conclusion: We present the first landscaping of the genome-wide binding pattern of TLX1 and provide evidence for a previously unestablished role of lncRNAs in the TLX1 regulatory network. Citation Format: Kaat Durinck, Wouter Van Loocke, Inge Van de Walle, Joni Van der Meulen, Pieter-Jan Volders, Nadine Van Roy, Yves Benoit, Bruce Poppe, Pieter Mestdagh, Jo Vandesompele, Pieter Rondou, Tom Taghon, Jean Soulier, Pieter Van Vlierberghe, Frank Speleman. Expanding the TLX1 regulome in T-cell acute lymphoblastic leukemia towards long noncoding RNAs. [abstract]. In: Proceedings of the AACR Special Conference on Chromatin and Epigenetics in Cancer; Sep 24-27, 2015; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2016;76(2 Suppl):Abstract nr A28." @default.
• W2400720319 created "2016-06-24" @default.
• W2400720319 creator A5000479325 @default.
• W2400720319 creator A5001469388 @default.
• W2400720319 creator A5015213142 @default.
• W2400720319 creator A5017485109 @default.
• W2400720319 creator A5023346779 @default.
• W2400720319 creator A5029983700 @default.
• W2400720319 creator A5057368279 @default.
• W2400720319 creator A5058438834 @default.
• W2400720319 creator A5059398199 @default.
• W2400720319 creator A5060684280 @default.
• W2400720319 creator A5062808434 @default.
• W2400720319 creator A5063657587 @default.
• W2400720319 creator A5078982772 @default.
• W2400720319 creator A5082071621 @default.
• W2400720319 creator A5086580140 @default.
• W2400720319 date "2016-01-14" @default.
• W2400720319 modified "2023-09-27" @default.
• W2400720319 title "Abstract A28: Expanding the TLX1 regulome in T-cell acute lymphoblastic leukemia towards long noncoding RNAs" @default.
• W2400720319 doi "https://doi.org/10.1158/1538-7445.chromepi15-a28" @default.
• W2400720319 hasPublicationYear "2016" @default.
• W2400720319 type Work @default.
• W2400720319 sameAs 2400720319 @default.
• W2400720319 citedByCount "0" @default.
• W2400720319 crossrefType "proceedings-article" @default.
• W2400720319 hasAuthorship W2400720319A5000479325 @default.
• W2400720319 hasAuthorship W2400720319A5001469388 @default.
• W2400720319 hasAuthorship W2400720319A5015213142 @default.
• W2400720319 hasAuthorship W2400720319A5017485109 @default.
• W2400720319 hasAuthorship W2400720319A5023346779 @default.
• W2400720319 hasAuthorship W2400720319A5029983700 @default.
• W2400720319 hasAuthorship W2400720319A5057368279 @default.
• W2400720319 hasAuthorship W2400720319A5058438834 @default.
• W2400720319 hasAuthorship W2400720319A5059398199 @default.
• W2400720319 hasAuthorship W2400720319A5060684280 @default.
• W2400720319 hasAuthorship W2400720319A5062808434 @default.
• W2400720319 hasAuthorship W2400720319A5063657587 @default.
• W2400720319 hasAuthorship W2400720319A5078982772 @default.
• W2400720319 hasAuthorship W2400720319A5082071621 @default.
• W2400720319 hasAuthorship W2400720319A5086580140 @default.
• W2400720319 hasConcept C104317684 @default.
• W2400720319 hasConcept C121587040 @default.
• W2400720319 hasConcept C150194340 @default.
• W2400720319 hasConcept C2778461978 @default.
• W2400720319 hasConcept C2909962599 @default.
• W2400720319 hasConcept C502942594 @default.
• W2400720319 hasConcept C54355233 @default.
• W2400720319 hasConcept C71924100 @default.
• W2400720319 hasConcept C86803240 @default.
• W2400720319 hasConceptScore W2400720319C104317684 @default.
• W2400720319 hasConceptScore W2400720319C121587040 @default.
• W2400720319 hasConceptScore W2400720319C150194340 @default.
• W2400720319 hasConceptScore W2400720319C2778461978 @default.
• W2400720319 hasConceptScore W2400720319C2909962599 @default.
• W2400720319 hasConceptScore W2400720319C502942594 @default.
• W2400720319 hasConceptScore W2400720319C54355233 @default.
• W2400720319 hasConceptScore W2400720319C71924100 @default.
• W2400720319 hasConceptScore W2400720319C86803240 @default.
• W2400720319 hasLocation W24007203191 @default.
• W2400720319 hasOpenAccess W2400720319 @default.
• W2400720319 hasPrimaryLocation W24007203191 @default.
• W2400720319 hasRelatedWork W1575982662 @default.
• W2400720319 hasRelatedWork W2087307929 @default.
• W2400720319 hasRelatedWork W2241514030 @default.
• W2400720319 hasRelatedWork W2297588567 @default.
• W2400720319 hasRelatedWork W2308641807 @default.
• W2400720319 hasRelatedWork W2406652818 @default.
• W2400720319 hasRelatedWork W2544623913 @default.
• W2400720319 hasRelatedWork W2563416939 @default.
• W2400720319 hasRelatedWork W2578419642 @default.
• W2400720319 hasRelatedWork W2588913130 @default.
• W2400720319 hasRelatedWork W2598712600 @default.
• W2400720319 hasRelatedWork W2725983537 @default.
• W2400720319 hasRelatedWork W2771936594 @default.
• W2400720319 hasRelatedWork W2912747319 @default.
• W2400720319 hasRelatedWork W3016564598 @default.
• W2400720319 hasRelatedWork W3023132905 @default.
• W2400720319 hasRelatedWork W3043544931 @default.
• W2400720319 hasRelatedWork W3107932947 @default.
• W2400720319 hasRelatedWork W3182601916 @default.
• W2400720319 hasRelatedWork W3207996117 @default.
• W2400720319 isParatext "false" @default.
• W2400720319 isRetracted "false" @default.
• W2400720319 magId "2400720319" @default.
• W2400720319 workType "article" @default.