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• W2400768500 abstract "Human articular chondrocytes (HAC) were cultivated as a monolayer in a serum-free medium for primary culture (SFM-P). An optimized SFM-P provides 95% proliferation rate of that obtainable from primary and secondary chondrocyte cultures grown in a control medium with serum. The gradual decrease in the amounts of synthesized glycosaminoglycan and type II collagen was improved by coating the culture dishes with type IV collagen and fibronectin. A significant improvement in the expression of type II collagen and aggrecan mRNA could be achieved. In addition, the monolayer cultures showed better synthesis of the extracellular matrices than alginate-bead cultures in SFM-P." @default.
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• W2400768500 date "2005-01-01" @default.
• W2400768500 modified "2023-10-17" @default.
• W2400768500 title "Development of serum-free media for primary culture of human articular chondrocytes" @default.
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