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• W2400812912 endingPage "220" @default.
• W2400812912 startingPage "205" @default.
• W2400812912 abstract "Targeting-induced local lesions in genomes (TILLING) is a general strategy for identifying induced point mutations that can be applied to almost any organism. Here, we describe the basic methodology for high-throughput TILLING. Gene segments are amplified using fluorescently tagged primers, and products are denatured and reannealed to form heteroduplexes between the mutated sequence and its wild-type counterpart. These heteroduplexes are substrates for cleavage by the endonuclease CEL I. Following cleavage, products are analyzed on denaturing polyacrylamide gels using the LI-COR DNA analyzer system. High-throughput TILLING has been adopted by the Arabidopsis TILLING Project (ATP) to provide allelic series of point mutations for the general Arabidopsis community." @default.
• W2400812912 created "2016-06-24" @default.
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• W2400812912 date "2003-11-15" @default.
• W2400812912 modified "2023-10-18" @default.
• W2400812912 title "High-Throughput TILLING for Functional Genomics" @default.
• W2400812912 cites W1516768294 @default.
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• W2400812912 doi "https://doi.org/10.1385/1-59259-413-1:205" @default.
• W2400812912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14501067" @default.
• W2400812912 hasPublicationYear "2003" @default.
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