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- W2400982485 abstract "AIM: To investigate whether insulin exerts protective effects against hepatocytes injury indirectly induced by LPS and if so, the mechanism involved. METHODS: Kupffer cells and hepatocytes were isolated from male Sprague-Dawley rats by pronasecollagenase perfusion and cultured. Kupffer cells were stimulated by lipopolysaccharide (LPS) or LPS+insulin for 4 h, the supernatant of different groups were collected to detect TNF-alpha and IL-10 by ELISA and to stimulate the primary cultured rat hepatocytes with or without anti-TNF-alpha antibody. After the hepatocytes were stimulated for 12 h, cell survival rate and injury indexes were detected. RESULTS: (1) The supernatant of LPS-activated Kupffer cells caused significant hepatocytes injury. Treatment with insulin produced a potent protective effect as evidenced by decreased ALT and AST levels (P<0.01, n=8) and improved hepatocytes survival rate (P<0.01, n=8). (2) Compared with the LPS group, insulin significantly decreased TNF-alpha secretion (P<0.01, n=8) and increased IL-10 secretion (P<0.05, n=8) from Kupffer cell stimulated by LPS. (3) Incubating hepatocytes with anti-TNF-alpha antibody attenuated hepatocytes injury induced by the supernatant of LPS-activated Kupffer cells. But treatment with anti-TNF-alpha antibody and insulin did not exert additive effect compared with treatment with insulin alone. CONCLUSION: Insulin has protective effects on rat hepatocytes injury indirectly induced by LPS, which is associated with suppression of the pro-inflammatory cytokine TNF-alpha and increment of the anti-inflammatory cytokine IL-10 both from Kupffer cells." @default.
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- W2400982485 date "2006-05-01" @default.
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- W2400982485 title "[Insulin prevents injury and enhances survival of rat hepatocytes via its anti-inflammatory effects]." @default.
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