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- W2401103616 abstract "We developed a strategy for covalent coupling of targeting proteins to liposomes decorated with a standard adapter protein. This strategy is based on dock and lock the interactions between two mutated fragments of human RNase I, a 1-15-aa fragment with the R4C amino acid substitution, (Cys-tag), and a 21-127-aa fragment with the V118C substitution, (Ad-C). Upon binding to each other, Cys-tag and Ad-C spontaneously form a disulfide bond between the complimentary 4C and 118C residues. Therefore, any targeting protein expressed with Cys-tag can be easily coupled to liposomes decorated with Ad-C. Here, we describe the preparation of Ad-liposomes followed by coupling them to two Cys-tagged targeted proteins, human vascular endothelial growth factor expressed with N-terminal Cys-tag, and a 254-aa long N-terminal fragment of anthrax lethal factor carrying C-terminal Cys-tag. Both proteins retain functional activity after coupling to Ad-C-decorated drug-loaded liposomes. We expect that our dock and lock strategy will open new opportunities for development of targeted therapeutic liposomes for research and clinical use." @default.
- W2401103616 created "2016-06-24" @default.
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- W2401103616 date "2009-11-21" @default.
- W2401103616 modified "2023-10-09" @default.
- W2401103616 title "A “Dock and Lock” Approach to Preparation of Targeted Liposomes" @default.
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- W2401103616 doi "https://doi.org/10.1007/978-1-60327-360-2_17" @default.
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