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- W2401233607 abstract "AIM: To construct regulable DNA vaccine against Plasmodium falciparum by using tetracycline(Tet) regulable system. METHODS: Eukaryotic expression vectors pTL 8/apical membrane antigen 1 (AMA 1) (tTA) and pTL 8/AMA 1(rtTA)gene which express trans activator (tTA) or reverse trans activator(rtTA), respectively, and AMA 1 gene of Plasmodium falciparum were constructed. BALB/c mice were immunized with these plasmids and doxycycline (dox) was administered to regulate the expression of AMA 1. For some mice immunized with pTL 8/AMA 1(rtTA), pUHS6 1, a plasmid containing trans silencer (tTS) to suppress basal expression of AMA 1 from pTL 8/AMA 1(rtTA), was injected into these mice together with pTL 8/AMA 1(rtTA). The sera of the mice were isolated at 2,4,6 and 8 weeks post immunization and the antibodies specific to AMA 1 were measured by ELISA. RESULTS: pTL 8/AMA 1 and pTL 8/AMA 1(rtTA) were constructed successfully. The mice immunized by pTL 8/AMA 1(tTA) with dox or by pTL 8/AMA 1(rtTA) without dox (at these conditions, AMA 1 was expressed at basal level)developed significant antibodies against AMA 1. Mice immunized by pTL 8/AMA 1(rtTA)and pUHS6 1 without dox did not develop significantly antibodies against AMA 1. In contrast, the mice immunized by pTL 8/AMA 1(rtTA)and pUHS6 1 with dox produced high level of antibodies. CONCLUSION: pTL 8/AMA 1(rtTA)combined with pUHS6 1 is a good regulable DNA vaccine candidate against Plasmodium falciparum ." @default.
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- W2401233607 date "2004-01-01" @default.
- W2401233607 modified "2023-09-23" @default.
- W2401233607 title "Preliminary study on regulable DNA vaccines against Plasmodium falciparum" @default.
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