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- W2401271421 abstract "The histidine biosynthesis gene (his) sequence was obtained from wheat-biotype Phaeosphaeria nodorum genomic DNA using a ”step down” PCR amplification technique. The 2700-bp his gene fragment contained two exons and a 51-bp intron. The two exons of this gene encoded a complete 881-amino acid protein. Like the histidine synthesis proteins in other filamentous ascomycetes, the deduced protein contained the conserved domains for three biosynthetic activities: phosphoribosyl-AMP cyclohydrolase (PRA-CH; EC 3.5.4.19), phosphoribosyl-ATP pyrophosphohydrolase (PRA-PH; EC 3.6.1.31), and histidinol dehydrogenase (HDH; EC 1.1.1.23). The substrate and zinc ion binding location in this tri-functional histidine biosynthesis protein is discussed." @default.
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- W2401271421 date "2006-03-01" @default.
- W2401271421 modified "2023-09-24" @default.
- W2401271421 title "The tri-functional histidine biosynthesis gene (his) in wheat stagonospora nodorum blotch pathogen, Phaeosphaeria nodorum." @default.
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