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• W2401298957 abstract "Microbial mutualisms play critical roles in a diverse number of ecosystems and have the potential to improve the efficiency of bioproduction for desirable chemicals. We investigate the growth of a photosynthetic cyanobacterium, Synechococcus elongatus PCC 7942, and a diazotroph, Azotobacter vinelandii, in coculture. From initial studies of the coculture grown in media with glutamate, we proposed a model of cross-feeding between these organisms. We then engineer a new microbial mutualism between Azotobacter vinelandii AV3 and cscB Synechococcus elongatus that grows in the absence of fixed carbon or nitrogen. The coculture cannot grow in the absence of a sucrose-exporting S. elongatus, and neither organism can grow alone without fixed carbon or nitrogen. This new system has the potential to produce industrially relevant products, such as polyhydroxybutyrate (PHB) and alginate, from air, water, phosphate, trace metals, and sunlight. We demonstrate the ability of the coculture to produce PHB in this work." @default.
• W2401298957 created "2016-06-24" @default.
• W2401298957 creator A5027760459 @default.
• W2401298957 creator A5038514520 @default.
• W2401298957 date "2016-06-06" @default.
• W2401298957 modified "2023-10-02" @default.
• W2401298957 title "A Designed <i>A. vinelandii</i>–<i>S. elongatus</i> Coculture for Chemical Photoproduction from Air, Water, Phosphate, and Trace Metals" @default.
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• W2401298957 doi "https://doi.org/10.1021/acssynbio.6b00107" @default.
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• W2401298957 hasPublicationYear "2016" @default.
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