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- W2401461197 abstract "DNA fragments were labeled with dinitrophenyl (DNP) residues by the reaction with 2,4-dinitrobenzaldehyde in alkaline condition and the labeled DNA was used as a probe for non-radioactive in situ hybridization. DNP-labeled DNA probes for T cell receptor beta chain, c-myc and HTLV-1 were hybridized in situ to mRNA on cell specimens fixed with Carnoy's fixative. DNA-mRNA hybrids were detected immunohistochemically using anti-DNP antibodies. Cytoplasms of adult T cell leukemia cells were stained with varied intensity when these probes were used. More than 70% of cells were positively stained with T cell receptor probe. However, less than 30% of cells were stained with c-myc and HTLV-1 probes. The present study indicates that non-radioactive in situ hybridization can be used for the characterization and classification of leukemia." @default.
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- W2401461197 date "1988-01-01" @default.
- W2401461197 modified "2023-09-27" @default.
- W2401461197 title "Use of non-radioactive DNA probes for the characterization of adult T-cell leukemia cells." @default.
- W2401461197 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3226923" @default.
- W2401461197 hasPublicationYear "1988" @default.
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