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• W2401474615 startingPage "209" @default.
• W2401474615 abstract "Moving past the discovery phase of proteomics, the term targeted proteomics combines multiple approaches investigating a certain set of proteins in more detail. One such targeted proteomics approach is the combination of liquid chromatography and selected or multiple reaction monitoring mass spectrometry (SRM, MRM). SRM-MS requires prior knowledge of the fragmentation pattern of peptides, as the presence of the analyte in a sample is determined by measuring the m/z values of predefined precursor and fragment ions. Using scheduled SRM-MS, many analytes can robustly be monitored allowing for high-throughput sample analysis of the same set of proteins over many conditions. In this chapter, fundaments of SRM-MS are explained as well as an optimized SRM pipeline from assay generation to data analyzed." @default.
• W2401474615 created "2016-06-24" @default.
• W2401474615 creator A5054449714 @default.
• W2401474615 date "2013-08-22" @default.
• W2401474615 modified "2023-09-27" @default.
• W2401474615 title "Selected Reaction Monitoring Mass Spectrometry: A Methodology Overview" @default.
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• W2401474615 doi "https://doi.org/10.1007/978-1-62703-631-3_16" @default.
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