FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2401807581> ?p ?o ?g. }

• W2401807581 endingPage "281" @default.
• W2401807581 startingPage "275" @default.
• W2401807581 abstract "Aspergillus awamori glucoamylase is a secreted glycoprotein containing N-linked carbohydrate recognition sites at Asn-171, Asn-182 and Asn-395. Site-directed mutagenesis was performed at Asn-182 and Asn-395 to determine whether these residues were N-glycosylated by Saccharomyces cerevisiae, to investigate the function of any glycans linked to them, and to determine the effect of their deamidation on glucoamylase thermostability. Asn-171 and Asn-395, but not Asn-182, were N-glycosylated. Deletion of the glycan N-linked to Asn-395 did not affect specific activity, but greatly decreased enzyme secretion and thermostability. The mutant lacking the N-glycan linked to Asn-395 was synthesized very slowly, and was more associated with cell membrane components and susceptible to proteinase degradation than were wild-type or other mutant glucoamylases. Its secreted form was 30-fold less thermostable than wild-type enzyme at pH 4.5. Replacement of Asn-182 by Gln to eliminate deamidation at this site did not change glucoamylase specific activity or thermostability, while replacement by Asp decreased specific activity about 25%, but increased thermostability moderately at pH 4.5 below 70 degrees C. Both mutations of Asn-182 increased glucoamylase production." @default.
• W2401807581 created "2016-06-24" @default.
• W2401807581 creator A5016172590 @default.
• W2401807581 creator A5023977346 @default.
• W2401807581 creator A5042669159 @default.
• W2401807581 date "1994-07-01" @default.
• W2401807581 modified "2023-10-18" @default.
• W2401807581 title "Substitution of asparagine residues in <i>Aspergillus awamori</i> glucoamylase by site-directed mutagenesis to eliminate N-glycosylation and inactivation by deamidation" @default.
• W2401807581 cites W1030517862 @default.
• W2401807581 cites W1131515033 @default.
• W2401807581 cites W1496656839 @default.
• W2401807581 cites W1532310879 @default.
• W2401807581 cites W1546595715 @default.
• W2401807581 cites W1573653133 @default.
• W2401807581 cites W1591676294 @default.
• W2401807581 cites W1908773263 @default.
• W2401807581 cites W1955275385 @default.
• W2401807581 cites W1967051522 @default.
• W2401807581 cites W1973782344 @default.
• W2401807581 cites W1981274345 @default.
• W2401807581 cites W2001019297 @default.
• W2401807581 cites W2007231835 @default.
• W2401807581 cites W2018018109 @default.
• W2401807581 cites W2023984641 @default.
• W2401807581 cites W2040118836 @default.
• W2401807581 cites W2049122170 @default.
• W2401807581 cites W2056749293 @default.
• W2401807581 cites W2076683612 @default.
• W2401807581 cites W2078689274 @default.
• W2401807581 cites W2087331627 @default.
• W2401807581 cites W2096057606 @default.
• W2401807581 cites W2101971141 @default.
• W2401807581 cites W2144219732 @default.
• W2401807581 cites W2169988510 @default.
• W2401807581 cites W2340208317 @default.
• W2401807581 cites W2888511473 @default.
• W2401807581 cites W31465123 @default.
• W2401807581 cites W4232534952 @default.
• W2401807581 cites W42925865 @default.
• W2401807581 doi "https://doi.org/10.1042/bj3010275" @default.
• W2401807581 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1137172" @default.
• W2401807581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8037681" @default.
• W2401807581 hasPublicationYear "1994" @default.
• W2401807581 type Work @default.
• W2401807581 sameAs 2401807581 @default.
• W2401807581 citedByCount "52" @default.
• W2401807581 countsByYear W24018075812013 @default.
• W2401807581 countsByYear W24018075812014 @default.
• W2401807581 countsByYear W24018075812015 @default.
• W2401807581 countsByYear W24018075812017 @default.
• W2401807581 countsByYear W24018075812018 @default.
• W2401807581 countsByYear W24018075812019 @default.
• W2401807581 countsByYear W24018075812020 @default.
• W2401807581 countsByYear W24018075812021 @default.
• W2401807581 countsByYear W24018075812022 @default.
• W2401807581 crossrefType "journal-article" @default.
• W2401807581 hasAuthorship W2401807581A5016172590 @default.
• W2401807581 hasAuthorship W2401807581A5023977346 @default.
• W2401807581 hasAuthorship W2401807581A5042669159 @default.
• W2401807581 hasBestOaLocation W24018075812 @default.
• W2401807581 hasConcept C103408237 @default.
• W2401807581 hasConcept C104317684 @default.
• W2401807581 hasConcept C108625454 @default.
• W2401807581 hasConcept C143065580 @default.
• W2401807581 hasConcept C153911025 @default.
• W2401807581 hasConcept C16318435 @default.
• W2401807581 hasConcept C181199279 @default.
• W2401807581 hasConcept C185592680 @default.
• W2401807581 hasConcept C206212055 @default.
• W2401807581 hasConcept C2775895851 @default.
• W2401807581 hasConcept C2777313579 @default.
• W2401807581 hasConcept C2780784506 @default.
• W2401807581 hasConcept C2781216036 @default.
• W2401807581 hasConcept C2909003921 @default.
• W2401807581 hasConcept C55493867 @default.
• W2401807581 hasConcept C69034178 @default.
• W2401807581 hasConcept C86803240 @default.
• W2401807581 hasConceptScore W2401807581C103408237 @default.
• W2401807581 hasConceptScore W2401807581C104317684 @default.
• W2401807581 hasConceptScore W2401807581C108625454 @default.
• W2401807581 hasConceptScore W2401807581C143065580 @default.
• W2401807581 hasConceptScore W2401807581C153911025 @default.
• W2401807581 hasConceptScore W2401807581C16318435 @default.
• W2401807581 hasConceptScore W2401807581C181199279 @default.
• W2401807581 hasConceptScore W2401807581C185592680 @default.
• W2401807581 hasConceptScore W2401807581C206212055 @default.
• W2401807581 hasConceptScore W2401807581C2775895851 @default.
• W2401807581 hasConceptScore W2401807581C2777313579 @default.
• W2401807581 hasConceptScore W2401807581C2780784506 @default.
• W2401807581 hasConceptScore W2401807581C2781216036 @default.
• W2401807581 hasConceptScore W2401807581C2909003921 @default.
• W2401807581 hasConceptScore W2401807581C55493867 @default.
• W2401807581 hasConceptScore W2401807581C69034178 @default.
• W2401807581 hasConceptScore W2401807581C86803240 @default.
• W2401807581 hasIssue "1" @default.
• W2401807581 hasLocation W24018075811 @default.
• W2401807581 hasLocation W24018075812 @default.
• W2401807581 hasLocation W24018075813 @default.

• next