Matches in SemOpenAlex for { <https://semopenalex.org/work/W2401975089> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2401975089 endingPage "52" @default.
- W2401975089 startingPage "549" @default.
- W2401975089 abstract "To mutate human annexin V gene and transform it to Pichia Pastoris for mutant human annexin V expression, so as to be purified as active annexin V with endogenous metal chelating site.The 5' and 3' end of native annexin V gene were mutated by specific primers. The mutant annexin V gene was inserted into pPIC9K and sequenced. The correct plasmid was linearized and transformed into Pichia Pastoris strain GS115 by electroporation. The transformants were selected from MD plates and cultured in BMGY medium and induced with methanol. The culture was centrifuged and the supernatant was analyzed by SDS-PAGE and silver staining. The binding activity of mutant human annexin V from culture supernatant was determined with phosphatidylserine exposed erythrocytes and fluorescein isothiocyanate-annexin V.The 5' end of native human annexin V gene was fused with GCAGGCGGCTGCGGCCAT coding sequence and 3' end 946-948 site TGT was mutated to AGC. Pichia Pastoris transformants secreted proteins of relative molecular mass 36 000 48 h after methanol induction. The concentration of this protein that inhibited 50% of the binding of fluorescein-annexin V was 4nmol/L.Highly-active recombinant mutant human annexin V with endogenous metal-chelating sites can be expressed in Pichia Pastoris system." @default.
- W2401975089 created "2016-06-24" @default.
- W2401975089 creator A5024621945 @default.
- W2401975089 creator A5028351634 @default.
- W2401975089 creator A5032346670 @default.
- W2401975089 creator A5068791457 @default.
- W2401975089 creator A5083084733 @default.
- W2401975089 date "2006-08-01" @default.
- W2401975089 modified "2023-09-26" @default.
- W2401975089 title "[Recombinant expression of mutant human annexin V]." @default.
- W2401975089 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16995311" @default.
- W2401975089 hasPublicationYear "2006" @default.
- W2401975089 type Work @default.
- W2401975089 sameAs 2401975089 @default.
- W2401975089 citedByCount "0" @default.
- W2401975089 crossrefType "journal-article" @default.
- W2401975089 hasAuthorship W2401975089A5024621945 @default.
- W2401975089 hasAuthorship W2401975089A5028351634 @default.
- W2401975089 hasAuthorship W2401975089A5032346670 @default.
- W2401975089 hasAuthorship W2401975089A5068791457 @default.
- W2401975089 hasAuthorship W2401975089A5083084733 @default.
- W2401975089 hasConcept C104317684 @default.
- W2401975089 hasConcept C143065580 @default.
- W2401975089 hasConcept C153911025 @default.
- W2401975089 hasConcept C185592680 @default.
- W2401975089 hasConcept C186852380 @default.
- W2401975089 hasConcept C2781372317 @default.
- W2401975089 hasConcept C40767141 @default.
- W2401975089 hasConcept C553184892 @default.
- W2401975089 hasConcept C55493867 @default.
- W2401975089 hasConcept C86803240 @default.
- W2401975089 hasConcept C88634738 @default.
- W2401975089 hasConceptScore W2401975089C104317684 @default.
- W2401975089 hasConceptScore W2401975089C143065580 @default.
- W2401975089 hasConceptScore W2401975089C153911025 @default.
- W2401975089 hasConceptScore W2401975089C185592680 @default.
- W2401975089 hasConceptScore W2401975089C186852380 @default.
- W2401975089 hasConceptScore W2401975089C2781372317 @default.
- W2401975089 hasConceptScore W2401975089C40767141 @default.
- W2401975089 hasConceptScore W2401975089C553184892 @default.
- W2401975089 hasConceptScore W2401975089C55493867 @default.
- W2401975089 hasConceptScore W2401975089C86803240 @default.
- W2401975089 hasConceptScore W2401975089C88634738 @default.
- W2401975089 hasIssue "4" @default.
- W2401975089 hasLocation W24019750891 @default.
- W2401975089 hasOpenAccess W2401975089 @default.
- W2401975089 hasPrimaryLocation W24019750891 @default.
- W2401975089 hasRelatedWork W2082558823 @default.
- W2401975089 hasRelatedWork W2267635838 @default.
- W2401975089 hasRelatedWork W2303279450 @default.
- W2401975089 hasRelatedWork W2348451229 @default.
- W2401975089 hasRelatedWork W2349740044 @default.
- W2401975089 hasRelatedWork W2350813529 @default.
- W2401975089 hasRelatedWork W2352335130 @default.
- W2401975089 hasRelatedWork W2353801376 @default.
- W2401975089 hasRelatedWork W2359752233 @default.
- W2401975089 hasRelatedWork W2365667390 @default.
- W2401975089 hasRelatedWork W2367021391 @default.
- W2401975089 hasRelatedWork W2368970529 @default.
- W2401975089 hasRelatedWork W2375766688 @default.
- W2401975089 hasRelatedWork W2382393558 @default.
- W2401975089 hasRelatedWork W2387694908 @default.
- W2401975089 hasRelatedWork W2392977148 @default.
- W2401975089 hasRelatedWork W2394261110 @default.
- W2401975089 hasRelatedWork W2410243610 @default.
- W2401975089 hasRelatedWork W3141599275 @default.
- W2401975089 hasRelatedWork W3143117959 @default.
- W2401975089 hasVolume "28" @default.
- W2401975089 isParatext "false" @default.
- W2401975089 isRetracted "false" @default.
- W2401975089 magId "2401975089" @default.
- W2401975089 workType "article" @default.