Matches in SemOpenAlex for { <https://semopenalex.org/work/W2401996129> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2401996129 endingPage "7" @default.
- W2401996129 startingPage "413" @default.
- W2401996129 abstract "Rat liver plasma-membrane-bound arginase was investigated in order to obtain data regarding its physico-chemical properties. Arginase bound to plasma membrane presented a specific activity of 0.74 +/- 0.09 IU/mg for the fully-activated enzyme, the pH of maximum activity being 9.8. Maximum stability was recorded at two pH values, 7 and 10.5 respectively. Mn2+ activated the enzyme, while Cu2+ and Zn2+, and to a lesser extend Co2+, showed a strong inhibitory effect. Ca2+ and Mg2+ had no effect at the concentrations assayed. The influence of temperature was studied in the presence and in the absence of Mn2+. The enzyme was stable up to 65 degrees C in both cases. Membrane- bound arginase showed an activation energy of 11.5 +/- 1.4 Kcal/mol between 20 and 40 degrees C, and 13.3 +/- 2.5 Kcal/mol between 40 and 60 degrees C. The Q10 for the same temperature ranges were 1.78 and 1.9 respectively. The membrane-bound enzyme presented two different Michaelis constants, one with high affinity (2.05 +/- 0.73 mM) and the other with low affinity for arginine (130 +/- 27.2 mM). Solubilized arginase showed very similar values. Among all the structural analogous assayed, only L-canavanine proved to be substrate for arginase, with and L-arginine/L-canavanine hydrolysis ratio of 5.8 +/- 0.28. No reactivity was found between plasma-membrane-bound arginase and anti-rat liver arginase antibodies raised in rabbits." @default.
- W2401996129 created "2016-06-24" @default.
- W2401996129 creator A5009372799 @default.
- W2401996129 creator A5033008312 @default.
- W2401996129 creator A5043285884 @default.
- W2401996129 date "1991-12-01" @default.
- W2401996129 modified "2023-09-27" @default.
- W2401996129 title "Physico-chemical properties of hepatocyte plasma-membrane-bound arginase." @default.
- W2401996129 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1725742" @default.
- W2401996129 hasPublicationYear "1991" @default.
- W2401996129 type Work @default.
- W2401996129 sameAs 2401996129 @default.
- W2401996129 citedByCount "2" @default.
- W2401996129 countsByYear W24019961292016 @default.
- W2401996129 crossrefType "journal-article" @default.
- W2401996129 hasAuthorship W2401996129A5009372799 @default.
- W2401996129 hasAuthorship W2401996129A5033008312 @default.
- W2401996129 hasAuthorship W2401996129A5043285884 @default.
- W2401996129 hasConcept C164007495 @default.
- W2401996129 hasConcept C181199279 @default.
- W2401996129 hasConcept C185592680 @default.
- W2401996129 hasConcept C18903297 @default.
- W2401996129 hasConcept C202751555 @default.
- W2401996129 hasConcept C2776200302 @default.
- W2401996129 hasConcept C2777289219 @default.
- W2401996129 hasConcept C2777468819 @default.
- W2401996129 hasConcept C28212737 @default.
- W2401996129 hasConcept C41625074 @default.
- W2401996129 hasConcept C515207424 @default.
- W2401996129 hasConcept C55493867 @default.
- W2401996129 hasConcept C86803240 @default.
- W2401996129 hasConceptScore W2401996129C164007495 @default.
- W2401996129 hasConceptScore W2401996129C181199279 @default.
- W2401996129 hasConceptScore W2401996129C185592680 @default.
- W2401996129 hasConceptScore W2401996129C18903297 @default.
- W2401996129 hasConceptScore W2401996129C202751555 @default.
- W2401996129 hasConceptScore W2401996129C2776200302 @default.
- W2401996129 hasConceptScore W2401996129C2777289219 @default.
- W2401996129 hasConceptScore W2401996129C2777468819 @default.
- W2401996129 hasConceptScore W2401996129C28212737 @default.
- W2401996129 hasConceptScore W2401996129C41625074 @default.
- W2401996129 hasConceptScore W2401996129C515207424 @default.
- W2401996129 hasConceptScore W2401996129C55493867 @default.
- W2401996129 hasConceptScore W2401996129C86803240 @default.
- W2401996129 hasIssue "6" @default.
- W2401996129 hasLocation W24019961291 @default.
- W2401996129 hasOpenAccess W2401996129 @default.
- W2401996129 hasPrimaryLocation W24019961291 @default.
- W2401996129 hasRelatedWork W1968751589 @default.
- W2401996129 hasRelatedWork W1974847931 @default.
- W2401996129 hasRelatedWork W1985351094 @default.
- W2401996129 hasRelatedWork W1994503304 @default.
- W2401996129 hasRelatedWork W2038023105 @default.
- W2401996129 hasRelatedWork W2045973492 @default.
- W2401996129 hasRelatedWork W2053144709 @default.
- W2401996129 hasRelatedWork W2460314210 @default.
- W2401996129 hasRelatedWork W3193522338 @default.
- W2401996129 hasRelatedWork W993596079 @default.
- W2401996129 hasVolume "99" @default.
- W2401996129 isParatext "false" @default.
- W2401996129 isRetracted "false" @default.
- W2401996129 magId "2401996129" @default.
- W2401996129 workType "article" @default.