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• W2402040891 abstract "The tumor microenvironment participates in the regulation of tumor progression and influences treatment sensitivity. In breast cancer, it also may play a role in determining the fate of non-invasive lesions such as ductal carcinoma in situ (DCIS), a non-obligate precursor of invasive diseases, which is aggressively treated despite its indolent nature in many patients since no biomarkers are available to predict the progression of DCIS to invasive disease. In vitro models of stromal activation by breast tumor cells might provide clues as to specific stromal genes crucial for the transition from DCIS to invasive disease.normal human dermal fibroblasts (NHDF) were treated under serum-free conditions with cell culture media conditioned by breast cancer cell lines (SkBr3, MDA-MB-468, T47D) for 72 h and subjected to gene expression profiling with Illumina platform.TGM2, coding for a tissue transglutaminase, was identified as candidate gene for stromal activation. In public transcriptomic datasets of invasive breast tumors TGM2 expression proved to provide prognostic information. Conversely, its role as an early biosensor of tumor invasiveness needs to be further investigated by in situ analyses.Stromal TGM2 might probably be associated with precancerous evolution at earlier stages compared to DCIS." @default.
• W2402040891 created "2016-06-24" @default.
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• W2402040891 date "2016-05-18" @default.
• W2402040891 modified "2023-10-11" @default.
• W2402040891 title "Stromal Activation by Tumor Cells: An in Vitro Study in Breast Cancer" @default.
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• W2402040891 doi "https://doi.org/10.3390/microarrays5020010" @default.
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