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- W2402051207 abstract "[Objective] The study was to report the construction of plant virus expression vector pClYVV/CP/W and the expression of green fluorescent protein(GFP) with pClYVV/CP/W,and to develop effective plat virus vector for plant bioreactor to produce useful protein.[Method] A section of multiple cloning sites among NIb/CP genes in pClYVV genome and deoxyribonucleotide polylinker of cleavage recognition sequence containing viral protease NIa were cloned with infectivity full-length cDNA of clover yellow vein virus(ClYVV),and pClYVV/CP/W vector was constructed,GFP gene was inserted into pClYVV/CP/W to construct the pClYVV/CP/W/GFP vector.The transcription situation of recombinant virus clone was detected by RT-PCR,and targeted gene products expressed by recombinant virus clone were detected with western blot(WB).[Result] The broad bean seedling inoculated with pClYVV/CP/W/GFP expressed the same symptom as wild type ClYVV,morbidity was of 100%,the result showed that recombinant virus clone pClYVV/CP/W/GFP didn't suppress,insertion of foreign gene didn't destroy the open reading frame of pClYVV/CP/W.Foreign gene can keep living in F4 progeny virus genome steadily,recombinant virus clone pClYVV/CP/W/GFP could steadily express GFP in progeny virus at least.[Conclusion] The useful plant virus vector was provided for useful protein expressing." @default.
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- W2402051207 date "2009-01-01" @default.
- W2402051207 modified "2023-09-24" @default.
- W2402051207 title "Construction of plant virus expression vector pCIYVV/CP/W and expression of GFP." @default.
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