Matches in SemOpenAlex for { <https://semopenalex.org/work/W2402072783> ?p ?o ?g. }
- W2402072783 endingPage "4895" @default.
- W2402072783 startingPage "4885" @default.
- W2402072783 abstract "In sharp contrast to the single ORF of the Schizosaccharomyces pombe retrotransposon Tf1, retroviruses and most retrotransposons employ two different ORFs to separately encode the Gag and Pol proteins. The different ORFs are thought to allow for overexpression of the Gag protein relative to Pol protein presumed necessary for the assembly of functional retrovirus particles and virus-like particles (VLPs). The results of in vivo experiments designed to detect the transposition of Tf1 show that Tf1 is indeed active and can insert itself into the host genome via a true retrotransposition process. Thus, a paradox emerged between the lack of any obvious means of overexpressing Tf1 Gag protein and the demonstrated functionality of the element. Epitope tagging experiments described here confirm that the Tf1 large ORF is intact and that there is no translational or transcriptional mechanism used to overexpress the Tf1 Gag protein. In addition, we used sucrose gradients and antisera specific for Tf1 capsid (CA) and integrase (IN) to show that the Tf1 proteins do assemble into uniform populations of macromolecular particles that also cosediment with Tf1 reverse transcription products. This evidence suggests that Tf1 proteins form VLPs without using the previously described mechanisms that retroviruses and retrotransposons require to overexpress Gag proteins." @default.
- W2402072783 created "2016-06-24" @default.
- W2402072783 creator A5010051653 @default.
- W2402072783 creator A5035827560 @default.
- W2402072783 creator A5075536007 @default.
- W2402072783 date "1993-12-01" @default.
- W2402072783 modified "2023-09-25" @default.
- W2402072783 title "Novel gene expression mechanism in a fission yeast retroelement: Tf1 proteins are derived from a single primary translation product." @default.
- W2402072783 cites W1019447032 @default.
- W2402072783 cites W1490400638 @default.
- W2402072783 cites W1501294022 @default.
- W2402072783 cites W161344049 @default.
- W2402072783 cites W1695243715 @default.
- W2402072783 cites W1777456151 @default.
- W2402072783 cites W1815028569 @default.
- W2402072783 cites W1853845900 @default.
- W2402072783 cites W1896629372 @default.
- W2402072783 cites W1914738526 @default.
- W2402072783 cites W1933609030 @default.
- W2402072783 cites W1969737313 @default.
- W2402072783 cites W1973643623 @default.
- W2402072783 cites W1991738184 @default.
- W2402072783 cites W1999506587 @default.
- W2402072783 cites W2000254114 @default.
- W2402072783 cites W2004730835 @default.
- W2402072783 cites W2006132678 @default.
- W2402072783 cites W2018289835 @default.
- W2402072783 cites W2020461380 @default.
- W2402072783 cites W2022473619 @default.
- W2402072783 cites W2025920026 @default.
- W2402072783 cites W2035416711 @default.
- W2402072783 cites W2044297195 @default.
- W2402072783 cites W2057576679 @default.
- W2402072783 cites W2059366520 @default.
- W2402072783 cites W2065313571 @default.
- W2402072783 cites W2067296939 @default.
- W2402072783 cites W2074275408 @default.
- W2402072783 cites W2081406207 @default.
- W2402072783 cites W2089471930 @default.
- W2402072783 cites W2099481042 @default.
- W2402072783 cites W2101108802 @default.
- W2402072783 cites W2115895870 @default.
- W2402072783 cites W2210162472 @default.
- W2402072783 cites W28812364 @default.
- W2402072783 cites W3147308350 @default.
- W2402072783 cites W5404037 @default.
- W2402072783 cites W999244358 @default.
- W2402072783 doi "https://doi.org/10.1002/j.1460-2075.1993.tb06178.x" @default.
- W2402072783 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/413943" @default.
- W2402072783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8223497" @default.
- W2402072783 hasPublicationYear "1993" @default.
- W2402072783 type Work @default.
- W2402072783 sameAs 2402072783 @default.
- W2402072783 citedByCount "61" @default.
- W2402072783 countsByYear W24020727832013 @default.
- W2402072783 countsByYear W24020727832014 @default.
- W2402072783 countsByYear W24020727832015 @default.
- W2402072783 countsByYear W24020727832016 @default.
- W2402072783 countsByYear W24020727832017 @default.
- W2402072783 countsByYear W24020727832018 @default.
- W2402072783 countsByYear W24020727832020 @default.
- W2402072783 countsByYear W24020727832023 @default.
- W2402072783 crossrefType "journal-article" @default.
- W2402072783 hasAuthorship W2402072783A5010051653 @default.
- W2402072783 hasAuthorship W2402072783A5035827560 @default.
- W2402072783 hasAuthorship W2402072783A5075536007 @default.
- W2402072783 hasBestOaLocation W24020727832 @default.
- W2402072783 hasConcept C104317684 @default.
- W2402072783 hasConcept C141231307 @default.
- W2402072783 hasConcept C167625842 @default.
- W2402072783 hasConcept C2776519988 @default.
- W2402072783 hasConcept C2777576037 @default.
- W2402072783 hasConcept C2778431562 @default.
- W2402072783 hasConcept C2779429346 @default.
- W2402072783 hasConcept C2780530800 @default.
- W2402072783 hasConcept C47289529 @default.
- W2402072783 hasConcept C4918238 @default.
- W2402072783 hasConcept C54355233 @default.
- W2402072783 hasConcept C6881719 @default.
- W2402072783 hasConcept C7029365 @default.
- W2402072783 hasConcept C86803240 @default.
- W2402072783 hasConcept C95444343 @default.
- W2402072783 hasConceptScore W2402072783C104317684 @default.
- W2402072783 hasConceptScore W2402072783C141231307 @default.
- W2402072783 hasConceptScore W2402072783C167625842 @default.
- W2402072783 hasConceptScore W2402072783C2776519988 @default.
- W2402072783 hasConceptScore W2402072783C2777576037 @default.
- W2402072783 hasConceptScore W2402072783C2778431562 @default.
- W2402072783 hasConceptScore W2402072783C2779429346 @default.
- W2402072783 hasConceptScore W2402072783C2780530800 @default.
- W2402072783 hasConceptScore W2402072783C47289529 @default.
- W2402072783 hasConceptScore W2402072783C4918238 @default.
- W2402072783 hasConceptScore W2402072783C54355233 @default.
- W2402072783 hasConceptScore W2402072783C6881719 @default.
- W2402072783 hasConceptScore W2402072783C7029365 @default.
- W2402072783 hasConceptScore W2402072783C86803240 @default.
- W2402072783 hasConceptScore W2402072783C95444343 @default.
- W2402072783 hasIssue "12" @default.