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- W2402162890 abstract "Peroxynitrite, a potent albeit deleterious biological oxidant, was electrooxidized at a bare platinum electrode in a medium of simulated body fluid (SBF) to nitrite anion and molecular oxygen. The SBF, prepared in accordance with literature procedure, has a pH of 7.4 and an ionic strength of 0.16. In this medium, peroxynitrite was observed to undergo a diffusion-controlled (D=1.63 x 10(-5) cm2/s) oxidation process at a bare platinum electrode with a heterogeneous rate constant, k(s), of 0.029 cm/sec. The formal redox potential, Eo', of this biological oxidant was determined as 1.18 V with respect to saturated calomel electrode (SCE) in a 2e- oxidation process. The electrochemical response of the oxidation process, measured in unit of current, is observed to be concentration dependent with a limit of detection of 1.6 x 10(-7) M. The linear dynamic range of the observed current-concentration plot extends over the entire concentration range studied. These observations make this electrochemical method a feasible technique for quantitative determination of peroxynitrite in vivo and in vitro." @default.
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- W2402162890 date "2004-09-01" @default.
- W2402162890 modified "2023-09-24" @default.
- W2402162890 title "Electrooxidation of peroxynitrite in simulated body fluid at platinum electrode." @default.
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