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- W2402299337 endingPage "1891" @default.
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- W2402299337 abstract "ABSTRACT Cdc28 is a cyclin-dependent protein kinase of Saccharomyces cerevisiae that is required for the G1/S and G2/M transitions of the cell division cycle. All previously described cdc28 mutants aside from cdc28-1N arrest division specifically in the G1 phase. cdc28-1N arrests division in G2/mitosis. We show here that the cdc28-109 mutant exhibits a mixed cell division arrest at 37°C with cells in both the G1 and G2 phases. In order to identify proteins that functionally interact with Cdc28, we isolated mutants that are colethal with cdc28-109 at its permissive temperature. We describe here our phenotypic analysis of two such mutants, hsf1-82 and ydj1-10, that affect the heat shock transcription factor and a yeast dnaj-like protein chaperone, respectively. hsf1-82 and ydj1-10 temperaturesensitive mutants arrest the cell division cycle at several stages. However, one predominant class of cells in both mutants was arrested with a large bud and a single vertex of microtubules. Electron microscopic analysis of such hsf1-82 cells showed that they contained an unduplicated spindle pole body with an enlarged half-bridge. Twodimensional gel electrophoresis of total cell proteins revealed that the hsf1-82 cells were specifically defective in the expression of the Hsc82 and Hsp82 proteins. Furthermore, the hsf1-82 mutation was suppressed by the HSC82 gene on a multicopy plasmid that restored Hsc82 protein to high levels in these cells. These results show that Hsf1 is required for spindle pole body duplication at 37°C." @default.
- W2402299337 created "2016-06-24" @default.
- W2402299337 creator A5031147868 @default.
- W2402299337 creator A5055681542 @default.
- W2402299337 creator A5063065904 @default.
- W2402299337 date "1997-08-15" @default.
- W2402299337 modified "2023-10-03" @default.
- W2402299337 title "A yeast heat shock transcription factor (Hsf1) mutant is defective in both Hsc82/Hsp82 synthesis and spindle pole body duplication" @default.
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- W2402299337 cites W1749612483 @default.
- W2402299337 cites W181622860 @default.
- W2402299337 cites W1891781482 @default.
- W2402299337 cites W1963954263 @default.
- W2402299337 cites W1964931840 @default.
- W2402299337 cites W1966797426 @default.
- W2402299337 cites W1968744144 @default.
- W2402299337 cites W1973221130 @default.
- W2402299337 cites W1983956180 @default.
- W2402299337 cites W1985578429 @default.
- W2402299337 cites W1994523623 @default.
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- W2402299337 cites W2023482892 @default.
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- W2402299337 doi "https://doi.org/10.1242/jcs.110.16.1879" @default.
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