Matches in SemOpenAlex for { <https://semopenalex.org/work/W2402728189> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2402728189 endingPage "43" @default.
- W2402728189 startingPage "436" @default.
- W2402728189 abstract "The photoaffinity ligand of the delta opioid receptor Tyr-D-Thr-Gly-pN3Phe-Leu-Thr (azido-DTLET) was iodinated and purified by high performance liquid chromatography. Monoiodo-azido-DTLET displayed a high affinity (KD = 15 nM) and is selective (Kl mu/Kl delta = 9.8) for rat brain delta opioid receptors (for comparison, the corresponding values for tritiated azido-DTLET are KD = 1.66 nM and Kl mu/Kl delta = 27). On rat brain sections, the anatomical distribution of [125I]azido-DTLET binding sites revealed by autoradiography corresponds to that of delta receptors. On rat brain membrane homogenates and NG108-15 hybrid cells, UV irradiation of the receptor-ligand complex results in the irreversible binding to membrane proteins of 14% of the bound radioactivity Gel electrophoresis of [125I]azido-DTLET-labeled proteins followed by autoradiography shows a different pattern in rat brain and NG108-15 cells. In rat brain, labeling of two of these proteins, with molecular weights of 44,000 and 34,000, was inhibited by 30 nmol/liter of nonradioactive DTLET, a delta-selective ligand but not by the same concentration of [D-Ala2,N-Me-Phe4,Gly5-ol]-enkephalin, a mu-selective ligand. In NG108-15 cells, this 44-kDa protein was not visualized; the main band was at 33 kDa and disappeared in the presence of levorphanol." @default.
- W2402728189 created "2016-06-24" @default.
- W2402728189 creator A5011338417 @default.
- W2402728189 creator A5019273314 @default.
- W2402728189 creator A5022379929 @default.
- W2402728189 creator A5033031837 @default.
- W2402728189 creator A5057419092 @default.
- W2402728189 creator A5059145575 @default.
- W2402728189 creator A5067880948 @default.
- W2402728189 date "1988-10-01" @default.
- W2402728189 modified "2023-09-26" @default.
- W2402728189 title "Photoaffinity labeling of opioid delta receptors with an iodinated azido-ligand: [125I][D-Thr2,pN3Phe4,Leu5]enkephalyl-Thr6." @default.
- W2402728189 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2845246" @default.
- W2402728189 hasPublicationYear "1988" @default.
- W2402728189 type Work @default.
- W2402728189 sameAs 2402728189 @default.
- W2402728189 citedByCount "7" @default.
- W2402728189 countsByYear W24027281892016 @default.
- W2402728189 countsByYear W24027281892020 @default.
- W2402728189 crossrefType "journal-article" @default.
- W2402728189 hasAuthorship W2402728189A5011338417 @default.
- W2402728189 hasAuthorship W2402728189A5019273314 @default.
- W2402728189 hasAuthorship W2402728189A5022379929 @default.
- W2402728189 hasAuthorship W2402728189A5033031837 @default.
- W2402728189 hasAuthorship W2402728189A5057419092 @default.
- W2402728189 hasAuthorship W2402728189A5059145575 @default.
- W2402728189 hasAuthorship W2402728189A5067880948 @default.
- W2402728189 hasConcept C116084860 @default.
- W2402728189 hasConcept C116569031 @default.
- W2402728189 hasConcept C141087196 @default.
- W2402728189 hasConcept C170493617 @default.
- W2402728189 hasConcept C185592680 @default.
- W2402728189 hasConcept C2777803847 @default.
- W2402728189 hasConcept C2778581200 @default.
- W2402728189 hasConcept C2778750930 @default.
- W2402728189 hasConcept C2779248205 @default.
- W2402728189 hasConcept C2780679169 @default.
- W2402728189 hasConcept C2780815831 @default.
- W2402728189 hasConcept C2781063702 @default.
- W2402728189 hasConcept C2908540775 @default.
- W2402728189 hasConcept C55493867 @default.
- W2402728189 hasConcept C71240020 @default.
- W2402728189 hasConceptScore W2402728189C116084860 @default.
- W2402728189 hasConceptScore W2402728189C116569031 @default.
- W2402728189 hasConceptScore W2402728189C141087196 @default.
- W2402728189 hasConceptScore W2402728189C170493617 @default.
- W2402728189 hasConceptScore W2402728189C185592680 @default.
- W2402728189 hasConceptScore W2402728189C2777803847 @default.
- W2402728189 hasConceptScore W2402728189C2778581200 @default.
- W2402728189 hasConceptScore W2402728189C2778750930 @default.
- W2402728189 hasConceptScore W2402728189C2779248205 @default.
- W2402728189 hasConceptScore W2402728189C2780679169 @default.
- W2402728189 hasConceptScore W2402728189C2780815831 @default.
- W2402728189 hasConceptScore W2402728189C2781063702 @default.
- W2402728189 hasConceptScore W2402728189C2908540775 @default.
- W2402728189 hasConceptScore W2402728189C55493867 @default.
- W2402728189 hasConceptScore W2402728189C71240020 @default.
- W2402728189 hasIssue "4" @default.
- W2402728189 hasLocation W24027281891 @default.
- W2402728189 hasOpenAccess W2402728189 @default.
- W2402728189 hasPrimaryLocation W24027281891 @default.
- W2402728189 hasRelatedWork W1492230825 @default.
- W2402728189 hasRelatedWork W1980933101 @default.
- W2402728189 hasRelatedWork W1999971930 @default.
- W2402728189 hasRelatedWork W2079512085 @default.
- W2402728189 hasRelatedWork W2085891026 @default.
- W2402728189 hasRelatedWork W2111140267 @default.
- W2402728189 hasRelatedWork W2328717646 @default.
- W2402728189 hasRelatedWork W2402728189 @default.
- W2402728189 hasRelatedWork W2415149031 @default.
- W2402728189 hasRelatedWork W2620546587 @default.
- W2402728189 hasVolume "34" @default.
- W2402728189 isParatext "false" @default.
- W2402728189 isRetracted "false" @default.
- W2402728189 magId "2402728189" @default.
- W2402728189 workType "article" @default.