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- W2402833535 abstract "Background: Quantification of polar compounds such as chloroquine by revered-phase LC is a challenge because of poor retention and silanol interactions with stationary phase. Strong ion-pairing reagents added to mobile phases to improve reversed-phase retention and improve peak shape can be harmful for MS. Results: This new approach provides a rapid and sensitive method for the detection of chloroquine using hydrophilic interaction LC coupled to MS/MS (HILIC–MS/MS). Ammonium formate and formic acid were added to mobile phase to attain good peak shapes and the salified chloroquine as well retained in an HILIC column. Linearity, intra- and inter-day precision, accuracy, recovery, matrix effect and stability were evaluated during the validation process. Conclusion: The validated method has been successfully used in a PK study in miniature pigs, and paves way for future development." @default.
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- W2402833535 date "2016-06-01" @default.
- W2402833535 modified "2023-09-26" @default.
- W2402833535 title "Development and validation of an HILIC–MS/MS method by one-step precipitation for chloroquine in miniature pig plasma" @default.
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- W2402833535 doi "https://doi.org/10.4155/bio-2015-0032" @default.
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