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- W2403087811 abstract "For many years matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) has been used to rapidly identify whole bacteria.However,no consistent methodology exists for the rapid identification of bacteria cultured in liquid medium.We explore the use of MALDI-TOF MS analysis to rapidly identify cells cultured in liquid medium.We determined that 2,5-dihydroxybenzoic acid(DHB,50mg/ml,50%acetonitrile,0.1%TFA) to be the best matrix solution for MALDI-TOF MS analysis.The strains tested could be differentiated by the principal component analysis(PCA) and the main characteristic of mass peaks for each species were found in their mix cultured samples.The minimum number of cells for detection was 1.8×103.Our findings suggest that the MS techniques can be developed as an auxiliary method for rapidly and accurately identifying bacteria cultured in liquid media." @default.
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- W2403087811 date "2010-01-01" @default.
- W2403087811 modified "2023-09-25" @default.
- W2403087811 title "Whole-Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Rapid Identification of Bacteria Cultured in Liquid Media" @default.
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