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- W2403280676 abstract "The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD).PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS-like genes and characterize EndoSd.EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium.Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd." @default.
- W2403280676 created "2016-06-24" @default.
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- W2403280676 date "2016-06-01" @default.
- W2403280676 modified "2023-10-18" @default.
- W2403280676 title "EndoSd: an IgG glycan hydrolyzing enzyme inStreptococcus dysgalactiaesubspeciesdysgalactiae" @default.
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- W2403280676 doi "https://doi.org/10.2217/fmb.16.14" @default.
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