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- W2403607031 abstract "Objective To evaluate of the accuracy of domestic commercial HBV DNA real-time polymerase chain reaction kits.Methotis Using COBAS TaqMan HBV Test as refefence,we evaluate the accuracy of a domestic commercial HBV DNA real-time polymerase chain reaction kit(PG).Results Among the samples with viral load at the range of 10~1,10~2,10~3,10~4,10~5,10~6,10~7(IU/mL),the Coefficient of Correlation(r)between the result determined by domestic kit(PG)and those of Roche COBAS TaqMan HBV Test were:-0.08011,-0.05056,0.105642,0.312181,0.908046,0.866175,-0.23295,respectively;the percentage of false negative results were 60%,30%,33.3%,8.3%,0,0,0,respectively.Among the samples with viral load over than 10~7 (IU/ml),the result determined by PG is significantly lowcr.Conclusion The accuracy of PG is not satisfied,especially in those samples with viral load less than 10~4(IU/ml).A implication from these observation is that samples from patients received antiviral treatment should be tested by Roche COBAS TaqMan HBV Test.Key words: Hepatitis B virus; DNA; Polymerase chain reaction" @default.
- W2403607031 created "2016-06-24" @default.
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- W2403607031 date "2009-12-01" @default.
- W2403607031 modified "2023-09-24" @default.
- W2403607031 title "[Evaluation of the accuracy of domestic commercial HBV DNA real-time polymerase chain reaction kits using COBAS TaqMan HBV Test as reference]." @default.
- W2403607031 doi "https://doi.org/10.3760/cma.j.issn.1003-9279.2009.06.026" @default.
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