FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2403623280> ?p ?o ?g. }

• W2403623280 endingPage "785" @default.
• W2403623280 startingPage "784" @default.
• W2403623280 abstract "Since the discovery of insulin, many investigators have explored the possibility of developing a n effective method for its oral administration. Attempts t o secure absorption of insulin from the gastrointestinal tract using adjuvants have been unsuccessful from a therapeutic point of view, but it has become generally accepted that insulin can be absorbed from the intestine of mammals and some hypoglycaemic activity of the absorbed insulin observed (Laskowski et al., 1959; Inouye & Mars, 1962; Speth & Christian, 1963; Engel et al., 1968; Galloway & Root, 1972). Patel & Ryman (1976, 1977) and Dapergolas et al. (1976) have demonstrated that liposomally entrapped insulin, when given orally, produces hypoglycaemia in both diabetic and normal rats. Thus liposomes not only seem t o protect insulin from proteolytic degradation in the stomach and intestine, but also act as structurally organized vehicles with endocrine therapeutic potential. This has led us t o investigate whether absorption of liposomally entrapped insulin occurs in the intestine of normal man. Liposomes containing insulin were prepared by the method of Patel & Ryman (1976) with the following modifications. Neutral liposomes were prepared by dissolving egg phosphatidylcholine/cholesteroI (1 : 5, w/w) in chloroform. To the dry lipid was added a mixture of crystalline ox insulin (2mg/ml) and bovine serum albumin (1 mgiml), prepared in 10mM-potassium phosphate containing 80m~-NaC1, p H 7.4 (phosphate/saline buffer). Liposomes thus formed were sonicated for 8 min, with cooling, a t 4°C using a 19mm titanium probe at 0.15nm in an MSE 150W sonicator. The sonicated liposomes were passed through a Sepharose 6B column, equilibrated with the phosphate/saline buffer, and the lipid fractions with insulin associated were collected and stored under N2 at 4°C. Preparations were transported from London t o Glasgow by rail and were received on the day of preparation and used shortly afterwards. For this pilot study, volunteers were healthy male medical graduates, who acted as their own controls. Liposomally entrapped insulin (20-200 units) a t body temperature were administered quickly into the jejunum by syringe through a radio-opaque tube 1.5mm in diameter. This tube was taken orally, retained by a small mercury-filled terminal balloon and located in the jejunum by image-intensified fluoroscopy. Each intubation was performed between 08:30 and 09:30h, the subject having fasted for 10h previously. Samples of venous blood were withdrawn and collected in lithium-heparin tubes. Blood glucose was assayed by the glucose oxidase method, and plasma insulin by immunoassay using Wellcome reagents. In the first subject 22 units of liposomally entrapped insulin were administered intrajejunally and there appeared to be a small but progressive rise in concentration of plasma immunoreactive insulin, but no significant change in blood glucose concentration. The following five subjects were given up to 200 units of liposomallyentrapped insulin. Again a small increase in the concentration of plasma immunoreactive insulin was observed, but generally insignificant changes were seen in blood glucose concentration. Control liposomes (without insulin) had no effect upon the concentration of either plasma immunoreactive insulin or of blood glucose. We have estimated that about 1 of the administered dose of insulin appears in the plasma by 60min in our normal subjects. This study used a range of insulin doses known to be effective parenterally and has not yielded overt clinical hypoglycaemia. Nevertheless, indications are that insulin is taken up by normal males from the liposomal complexes, but presently acts a t too" @default.
• W2403623280 created "2016-06-24" @default.
• W2403623280 creator A5004204224 @default.
• W2403623280 creator A5020790094 @default.
• W2403623280 creator A5022035820 @default.
• W2403623280 creator A5022486146 @default.
• W2403623280 creator A5025760374 @default.
• W2403623280 creator A5060041747 @default.
• W2403623280 creator A5070261898 @default.
• W2403623280 creator A5088564216 @default.
• W2403623280 date "1978-08-01" @default.
• W2403623280 modified "2023-09-26" @default.
• W2403623280 title "Intrajejunal Absorption of Liposomally Entrapped Insulin in Normal Man" @default.
• W2403623280 cites W2463108427 @default.
• W2403623280 doi "https://doi.org/10.1042/bst0060784" @default.
• W2403623280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/710718" @default.
• W2403623280 hasPublicationYear "1978" @default.
• W2403623280 type Work @default.
• W2403623280 sameAs 2403623280 @default.
• W2403623280 citedByCount "24" @default.
• W2403623280 crossrefType "journal-article" @default.
• W2403623280 hasAuthorship W2403623280A5004204224 @default.
• W2403623280 hasAuthorship W2403623280A5020790094 @default.
• W2403623280 hasAuthorship W2403623280A5022035820 @default.
• W2403623280 hasAuthorship W2403623280A5022486146 @default.
• W2403623280 hasAuthorship W2403623280A5025760374 @default.
• W2403623280 hasAuthorship W2403623280A5060041747 @default.
• W2403623280 hasAuthorship W2403623280A5070261898 @default.
• W2403623280 hasAuthorship W2403623280A5088564216 @default.
• W2403623280 hasConcept C125287762 @default.
• W2403623280 hasConcept C126322002 @default.
• W2403623280 hasConcept C159985019 @default.
• W2403623280 hasConcept C185154212 @default.
• W2403623280 hasConcept C185592680 @default.
• W2403623280 hasConcept C192562407 @default.
• W2403623280 hasConcept C2777882243 @default.
• W2403623280 hasConcept C2779306644 @default.
• W2403623280 hasConcept C2780512811 @default.
• W2403623280 hasConcept C55493867 @default.
• W2403623280 hasConcept C71924100 @default.
• W2403623280 hasConcept C98274493 @default.
• W2403623280 hasConceptScore W2403623280C125287762 @default.
• W2403623280 hasConceptScore W2403623280C126322002 @default.
• W2403623280 hasConceptScore W2403623280C159985019 @default.
• W2403623280 hasConceptScore W2403623280C185154212 @default.
• W2403623280 hasConceptScore W2403623280C185592680 @default.
• W2403623280 hasConceptScore W2403623280C192562407 @default.
• W2403623280 hasConceptScore W2403623280C2777882243 @default.
• W2403623280 hasConceptScore W2403623280C2779306644 @default.
• W2403623280 hasConceptScore W2403623280C2780512811 @default.
• W2403623280 hasConceptScore W2403623280C55493867 @default.
• W2403623280 hasConceptScore W2403623280C71924100 @default.
• W2403623280 hasConceptScore W2403623280C98274493 @default.
• W2403623280 hasIssue "4" @default.
• W2403623280 hasLocation W24036232801 @default.
• W2403623280 hasLocation W24036232802 @default.
• W2403623280 hasOpenAccess W2403623280 @default.
• W2403623280 hasPrimaryLocation W24036232801 @default.
• W2403623280 hasRelatedWork W1976191247 @default.
• W2403623280 hasRelatedWork W2040746407 @default.
• W2403623280 hasRelatedWork W2091030955 @default.
• W2403623280 hasRelatedWork W2126187048 @default.
• W2403623280 hasRelatedWork W2372718936 @default.
• W2403623280 hasRelatedWork W2416203993 @default.
• W2403623280 hasRelatedWork W2473561882 @default.
• W2403623280 hasRelatedWork W2748952813 @default.
• W2403623280 hasRelatedWork W2899084033 @default.
• W2403623280 hasRelatedWork W47464580 @default.
• W2403623280 hasVolume "6" @default.
• W2403623280 isParatext "false" @default.
• W2403623280 isRetracted "false" @default.
• W2403623280 magId "2403623280" @default.
• W2403623280 workType "article" @default.