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- W2403934869 abstract "Publisher Summary This chapter describes genetic engineering which is the transfer of DNA between hosts by in vitro enzymatic manipulations. This implies that the DNA to be transferred is duplicated in the new host. As most DNA fragments are incapable of self-replication in E. coli or any other host cell, an additional segment of DNA, capable of autonomous replication, must be linked to the fragment to be cloned. This autonomously replicating fragment is the molecular cloning vector and plays a central role in recombinant DNA technology. Most cloning vectors have been originally derived from naturally occurring extrachromosomal elements, such as bacteriophage and plasmids. Bacteriophage vectors, such as M13 and lambda, have proven to be very useful as cloning vectors. Wild-type plasmids, such as pSCl0l and ColEl have served as two of the first cloning vectors. Although both plasmids possessed certain features that made them useful as vectors, there are no vectors available at the time that possessed all of these features in one plasmid." @default.
- W2403934869 created "2016-06-24" @default.
- W2403934869 creator A5000890370 @default.
- W2403934869 creator A5061749338 @default.
- W2403934869 creator A5073648973 @default.
- W2403934869 creator A5091755709 @default.
- W2403934869 date "1988-01-01" @default.
- W2403934869 modified "2023-09-25" @default.
- W2403934869 title "The Plasmid, pBR322" @default.
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