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- W2404060779 abstract "AIM: To express and purify Lugurus zone pellucida 3 (LZP3) in prokaryoti c cells and to prepare the LZP3-specific rabbit antiserum. METHODS: The core fragment of LZP3 gene was cloned into plasmid pGEX-4T -1 containing glutathione s-transferase(GST) fusion protein gene. Following re striction enzyme digestion analysis and sequencing, pGEX-4T-LZP3 w as transformed into E.coli BL21(DE3). GST-LZP3 fusion protein was expressed under IPTG induction and further purified with Glutathione Sepharose 4B. Then the purified GST-LZP3 fusion protein was used to immunize New Zealand rabbits. LZP3-specific rabbit antiserum was identified by ELISA and Western blot. RESULTS: GST-LZP3 fusion protein was overexpressed and its LZP3-spe cific antiserum was obtained. CONCLUSION: The successful express ion of GST-LZP3 fusion protein in E.coli and the preparation of LZP3-spec ific rabbit antiserum will be valuable for the study on birth control of Lagu rus." @default.
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- W2404060779 date "2004-03-01" @default.
- W2404060779 modified "2023-09-24" @default.
- W2404060779 title "Expression of Lagurus ZP3 fusion protein in prokaryotic cells and preparation of its antiserum" @default.
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