Matches in SemOpenAlex for { <https://semopenalex.org/work/W2404227542> ?p ?o ?g. }
- W2404227542 endingPage "250" @default.
- W2404227542 startingPage "241" @default.
- W2404227542 abstract "Abstract Bovine α-lactalbumin (ALA) is an important Ca-binding protein of milk. Epigallocatechin-3-gallate (EGCG) is the major and the most biologically active catechin of green tea, which has the highest binding affinity to whey proteins due to galloyl functional group. In this study experimental and computational methods were used to investigate noncovalent interactions of EGCG and ALA. Binding affinity of EGCG for ALA, determined by fluorescence quenching analysis, was in the range described for complexes of EGCG and other dietary proteins, and lower than affinity of some phenolic compounds to ALA. Based on circular dichroism and Fourier transform infrared spectroscopy spectra, binding of EGCG change ALA conformation inducing α-helix to β-structures transition. The isothermal titration calorimetry results suggest that the binding of EGCG to ALA is enthalpically favorable. The docking analysis shows that EGCG binds in the hydrophobic pocket at the entrance of cleft between α-helical and β-sheet-rich domains and includes residues of aromatic cluster II. Uptake of ALA by monocytes proceeds at a slower rate in the presence of EGCG suggesting that EGCG binding may impair uptake of ALA by antigen-presenting cells. ALA, being of low cost and widely available protein, can serve as suitable delivery system for EGCG, as well as for food fortification with this bioactive catechin." @default.
- W2404227542 created "2016-06-24" @default.
- W2404227542 creator A5011879949 @default.
- W2404227542 creator A5013309469 @default.
- W2404227542 creator A5025932691 @default.
- W2404227542 creator A5034994757 @default.
- W2404227542 creator A5056557106 @default.
- W2404227542 creator A5075439797 @default.
- W2404227542 creator A5078341152 @default.
- W2404227542 creator A5080380311 @default.
- W2404227542 creator A5088089298 @default.
- W2404227542 date "2016-12-01" @default.
- W2404227542 modified "2023-10-02" @default.
- W2404227542 title "Noncovalent interactions of bovine α-lactalbumin with green tea polyphenol, epigalocatechin-3-gallate" @default.
- W2404227542 cites W1597616608 @default.
- W2404227542 cites W1979348107 @default.
- W2404227542 cites W1981157132 @default.
- W2404227542 cites W1981164308 @default.
- W2404227542 cites W1982864607 @default.
- W2404227542 cites W1990622532 @default.
- W2404227542 cites W1991379964 @default.
- W2404227542 cites W1993695095 @default.
- W2404227542 cites W1996596782 @default.
- W2404227542 cites W2003049495 @default.
- W2404227542 cites W2004309451 @default.
- W2404227542 cites W2008304257 @default.
- W2404227542 cites W2008941128 @default.
- W2404227542 cites W2016421352 @default.
- W2404227542 cites W2017955861 @default.
- W2404227542 cites W2021583455 @default.
- W2404227542 cites W2023775627 @default.
- W2404227542 cites W2034710312 @default.
- W2404227542 cites W2038309937 @default.
- W2404227542 cites W2039133791 @default.
- W2404227542 cites W2055205074 @default.
- W2404227542 cites W2055565359 @default.
- W2404227542 cites W2056538157 @default.
- W2404227542 cites W2057687949 @default.
- W2404227542 cites W2059541587 @default.
- W2404227542 cites W2059635975 @default.
- W2404227542 cites W2060343376 @default.
- W2404227542 cites W2062277779 @default.
- W2404227542 cites W2064628455 @default.
- W2404227542 cites W2067436146 @default.
- W2404227542 cites W2067488430 @default.
- W2404227542 cites W2068989469 @default.
- W2404227542 cites W2071921237 @default.
- W2404227542 cites W2073179123 @default.
- W2404227542 cites W2073492763 @default.
- W2404227542 cites W2075988276 @default.
- W2404227542 cites W2076075465 @default.
- W2404227542 cites W2082014962 @default.
- W2404227542 cites W2091348010 @default.
- W2404227542 cites W2100837269 @default.
- W2404227542 cites W2101689484 @default.
- W2404227542 cites W2140347771 @default.
- W2404227542 cites W2144360365 @default.
- W2404227542 cites W2149148919 @default.
- W2404227542 cites W2151605986 @default.
- W2404227542 cites W2162166182 @default.
- W2404227542 cites W2168240189 @default.
- W2404227542 cites W2168677256 @default.
- W2404227542 cites W2331839753 @default.
- W2404227542 cites W400840232 @default.
- W2404227542 cites W4239393922 @default.
- W2404227542 doi "https://doi.org/10.1016/j.foodhyd.2016.05.012" @default.
- W2404227542 hasPublicationYear "2016" @default.
- W2404227542 type Work @default.
- W2404227542 sameAs 2404227542 @default.
- W2404227542 citedByCount "97" @default.
- W2404227542 countsByYear W24042275422016 @default.
- W2404227542 countsByYear W24042275422017 @default.
- W2404227542 countsByYear W24042275422018 @default.
- W2404227542 countsByYear W24042275422019 @default.
- W2404227542 countsByYear W24042275422020 @default.
- W2404227542 countsByYear W24042275422021 @default.
- W2404227542 countsByYear W24042275422022 @default.
- W2404227542 countsByYear W24042275422023 @default.
- W2404227542 crossrefType "journal-article" @default.
- W2404227542 hasAuthorship W2404227542A5011879949 @default.
- W2404227542 hasAuthorship W2404227542A5013309469 @default.
- W2404227542 hasAuthorship W2404227542A5025932691 @default.
- W2404227542 hasAuthorship W2404227542A5034994757 @default.
- W2404227542 hasAuthorship W2404227542A5056557106 @default.
- W2404227542 hasAuthorship W2404227542A5075439797 @default.
- W2404227542 hasAuthorship W2404227542A5078341152 @default.
- W2404227542 hasAuthorship W2404227542A5080380311 @default.
- W2404227542 hasAuthorship W2404227542A5088089298 @default.
- W2404227542 hasConcept C112887158 @default.
- W2404227542 hasConcept C137277065 @default.
- W2404227542 hasConcept C13965031 @default.
- W2404227542 hasConcept C178790620 @default.
- W2404227542 hasConcept C185592680 @default.
- W2404227542 hasConcept C2778004101 @default.
- W2404227542 hasConcept C2779431889 @default.
- W2404227542 hasConcept C2780295536 @default.
- W2404227542 hasConcept C2992814287 @default.
- W2404227542 hasConcept C31903555 @default.