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- W2404337083 abstract "Background : Simple manual method using a Nageotte hemocytometer counting residual leukocytes [White blood cells(WBC)] in blood components is subjective, labor-intensive, time consuming and variable within and between laboratories. The aim of this study was to evaluate usefulness for flow cytometric method to determinate very low numbers of leukocytes in leukocyte-free blood components. Methods: Epics XL-MCL (Beckman Coulter Co.) was used for determination of fluorescence-labelled cells. The DNA in leukocytes was stained using propidium iodide (PI) and leukocytes were automatically analysed by flow cytometer. Results : Linearity determined over a range of 0.5-50 WBC/ was a value of r=0.994. The detection limit of this method was 4.4 WBC/ and accuracy was 86.6% with linearity of r=0.991 over the 5-50 WBC/. Reproducibility was CV of 9.1% for 25.8 WBC/ and 14.7% for 7.1 WBC/, respectively. Conclusion : Flow cytometric techniques provide a reproducible and objective tool for counting residual WBC in leukocyte free blood components compared with the Nageotte hemocytometer." @default.
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- W2404337083 date "2004-01-01" @default.
- W2404337083 modified "2023-09-27" @default.
- W2404337083 title "Flow Cytometric Method for Counting Residual Leukocytes" @default.
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