FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2404534010> ?p ?o ?g. }

• W2404534010 endingPage "13" @default.
• W2404534010 startingPage "308" @default.
• W2404534010 abstract "To investigate the antitumour molecular mechanisms of WP1066 (STAT-3 inhibitor ) to human tongue squamous cell carcinoma in vitro and in vivo.WP1066 was used to inhibit the p-STAT-3 expression in Tscca human tongue squamous cell carcinoma cell line .Real-time PCR was used to detect the microRNA-21 expression after treatment with DMSO and WP1066. Methyl thiazolyl tatrozolium (MTT) assay was employed to determine cell survival. Flow cytometry (FCM) was used to measure apoptosis. The expression level of STAT-3/p-STAT-3, programmed cell death protein 4 (PDCD-4), antigen 67 (Ki-67), B cell lymphoma 2 (Bcl-2) and cleaved cysteinyl aspartate specific proteinase-3 (CCASP-3) was examined by Western blotting. Luciferase reporter gene assay was conducted to verify the regulation of STAT-3 to microRNA-21. Tscca xenograft tumor model was established in BALB/c nude mice and the tumors were divided into control, DMSO and WP1066 treated groups. The tumor tissues were measured by immunohistochemistry stain and terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) assay.STAT-3/p-STAT-3 protein was suppressed after treatment with WP1066 (STAT-3:F = 15.336, P = 0.004, p-STAT-3: F = 52.837, P = 0.000). MicroRNA-21 relative expression level was down-regulated (F = 8.197, P = 0.019). Cell survival rate was significantly reduced after treatment with WP1066 than control groups (F = 94.388, P = 0.000). Early apoptosis rate increased after treatment with WP1066 (F = 217.080, P = 0.000) . PDCD-4 and cleaved cysteinyl aspartate specific proteinase-3 (CCASP-3) protein expression was increased after treatment with WP1066 (PDCD-4:F = 8.771, P = 0.017; CCASP-3: F = 26.611, P = 0.001) .Ki-67 and Bcl-2 protein was down regulated (Ki-67:F = 5.854, P = 0.039; Bcl-2:F = 125.502, P = 0.000). Luciferase reporter gene assay proved that STAT-3 combined with specific promoter region of microRNA-21.In vivo, the tumor volume after treatment with WP1066 was significantly smaller than control groups by the end of observation (F = 15.390, P = 0.000) .Immunological histological chemistry indicated that PDCD-4 and CCASP-3 protein expression was up-regulated simultaneously while Ki-67 and Bcl-2 protein of tumor tissue was down-regulated after treatment with WP1066 than control groups. TUNEL assay suggested that apoptosis index rose after treatment with WP1066 than control groups (F = 133.368, P = 0.000) .WP1066 affected Tscca cancer cell proliferation and apoptosis via inhibiting STAT-3/microRNA-21.WP1066 provided new direction and possibility to human tongue squamous cell carcinoma therapy." @default.
• W2404534010 created "2016-06-24" @default.
• W2404534010 creator A5005311800 @default.
• W2404534010 creator A5009881958 @default.
• W2404534010 creator A5019290469 @default.
• W2404534010 creator A5030512618 @default.
• W2404534010 creator A5035752387 @default.
• W2404534010 creator A5086148274 @default.
• W2404534010 date "2014-05-01" @default.
• W2404534010 modified "2023-10-16" @default.
• W2404534010 title "[Signal transducer and activator of transcription-3 inhibitor WP1066 affects human tongue squamous cell carcinoma proliferation and apoptosis in vitro and in vivo]." @default.
• W2404534010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25031007" @default.
• W2404534010 hasPublicationYear "2014" @default.
• W2404534010 type Work @default.
• W2404534010 sameAs 2404534010 @default.
• W2404534010 citedByCount "2" @default.
• W2404534010 countsByYear W24045340102020 @default.
• W2404534010 countsByYear W24045340102022 @default.
• W2404534010 crossrefType "journal-article" @default.
• W2404534010 hasAuthorship W2404534010A5005311800 @default.
• W2404534010 hasAuthorship W2404534010A5009881958 @default.
• W2404534010 hasAuthorship W2404534010A5019290469 @default.
• W2404534010 hasAuthorship W2404534010A5030512618 @default.
• W2404534010 hasAuthorship W2404534010A5035752387 @default.
• W2404534010 hasAuthorship W2404534010A5086148274 @default.
• W2404534010 hasConcept C153911025 @default.
• W2404534010 hasConcept C171958077 @default.
• W2404534010 hasConcept C185592680 @default.
• W2404534010 hasConcept C190283241 @default.
• W2404534010 hasConcept C196795494 @default.
• W2404534010 hasConcept C2778923194 @default.
• W2404534010 hasConcept C502942594 @default.
• W2404534010 hasConcept C54355233 @default.
• W2404534010 hasConcept C553184892 @default.
• W2404534010 hasConcept C55493867 @default.
• W2404534010 hasConcept C62112901 @default.
• W2404534010 hasConcept C81885089 @default.
• W2404534010 hasConcept C86803240 @default.
• W2404534010 hasConceptScore W2404534010C153911025 @default.
• W2404534010 hasConceptScore W2404534010C171958077 @default.
• W2404534010 hasConceptScore W2404534010C185592680 @default.
• W2404534010 hasConceptScore W2404534010C190283241 @default.
• W2404534010 hasConceptScore W2404534010C196795494 @default.
• W2404534010 hasConceptScore W2404534010C2778923194 @default.
• W2404534010 hasConceptScore W2404534010C502942594 @default.
• W2404534010 hasConceptScore W2404534010C54355233 @default.
• W2404534010 hasConceptScore W2404534010C553184892 @default.
• W2404534010 hasConceptScore W2404534010C55493867 @default.
• W2404534010 hasConceptScore W2404534010C62112901 @default.
• W2404534010 hasConceptScore W2404534010C81885089 @default.
• W2404534010 hasConceptScore W2404534010C86803240 @default.
• W2404534010 hasIssue "5" @default.
• W2404534010 hasLocation W24045340101 @default.
• W2404534010 hasOpenAccess W2404534010 @default.
• W2404534010 hasPrimaryLocation W24045340101 @default.
• W2404534010 hasRelatedWork W2056570618 @default.
• W2404534010 hasRelatedWork W2283774595 @default.
• W2404534010 hasRelatedWork W2350466793 @default.
• W2404534010 hasRelatedWork W2351060232 @default.
• W2404534010 hasRelatedWork W2353723148 @default.
• W2404534010 hasRelatedWork W2358337505 @default.
• W2404534010 hasRelatedWork W2363045816 @default.
• W2404534010 hasRelatedWork W2391900612 @default.
• W2404534010 hasRelatedWork W3143982046 @default.
• W2404534010 hasRelatedWork W3148245999 @default.
• W2404534010 hasVolume "49" @default.
• W2404534010 isParatext "false" @default.
• W2404534010 isRetracted "false" @default.
• W2404534010 magId "2404534010" @default.
• W2404534010 workType "article" @default.