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- W2404594816 abstract "This study was performed to test whether the inhibitory effect of elevated D-glucose concentrations on insulin-stimulated chemokinesis in normal human neutrophils is mediated by increase in protein kinase C (PKC) activity. Activation of PKC with phorbol 12-myristate 13-acetate (PMA) at 0-100 nM dose-dependently inhibited neutrophil random locomotion in the absence of insulin. Sub-optimal concentrations of PMA (0.1-0.5 nM) inhibited the chemokinetic effect of 160 microU/mL insulin in a dose-dependent way. The specific PKC inhibitor bisindolylmaleimide (GF 109203x) did not affect the insulin-stimulated chemokinesis at 5 mM glucose but restored the chemokinetic effect of insulin at 15 mM glucose. These results therefore suggest that glucose-induced PKC activation may mediate the inhibitory effects of high glucose levels on insulin-stimulated chemokinesis in normal human neutrophils." @default.
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- W2404594816 date "1999-05-01" @default.
- W2404594816 modified "2023-10-17" @default.
- W2404594816 title "Hyperglycemia <i>in vitro</i> attenuates insulin-stimulated chemokinesis in normal human neutrophils. Role of protein kinase C activation" @default.
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- W2404594816 doi "https://doi.org/10.1002/jlb.65.5.635" @default.
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