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- W2404621857 abstract "Primordial germ cells (PGCs) play essential roles in both reproduction and development. In this chapter, we describe a method used in our laboratory for the immunopurification of PGCs from the mouse embryo. After dissection and disruption of the fetal gonad, PGCs are identified by a monoclonal antibody recognizing an epitope characteristic of pluripotent stem cells. After reaction with a paramagnetic bead-linked secondary antibody, the cell mixture is applied to a strong magnetic field. PGCs are recovered by release from the magnetic field. Purity is assessed by the alkaline phosphatase activity inherent to PGCs." @default.
- W2404621857 created "2016-06-24" @default.
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- W2404621857 date "2012-01-01" @default.
- W2404621857 modified "2023-09-23" @default.
- W2404621857 title "Immunomagnetic Purification of Murine Primordial Germ Cells" @default.
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- W2404621857 doi "https://doi.org/10.1007/978-1-62703-011-3_4" @default.
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